Relationship Analysis of Sibling Pairs on Madurese Ethnicity in Surabaya, Using 12 STR Loci for The Paternity Test Process

The Use of STR locus in studying the relations between siblings for the need of ethnic genetic database in Indonesia for identifying disaster victims, criminals and paternity test is not well established compared to other countries. Targeted outcomes are STR typing and profiling of the human genome for individual identification and kinship relations. The aim of this study was to analyse kinship relationships among siblings by assessing and determining a better STR locus and the level of allelic distribution among full siblings in Madurese people of Surabaya, East Java, with 3 kinship categories namely male-male, female-female and male-female. Objectives to explain the sibling relationship from a pool of 25 Madurese families in Surabaya, there were 6, 11 and 8 pairs of siblings in the male-male, female-female and male-female categories respectively. Kin relationships were analysed by using 12 STR loci technique (CSF1PO, F13B, FES, TH01, TPOX, VWA, D5S818, D7S820, D8S1179, D13S317, D16S539, and D18S51). Through the use of 12 autosomal STR loci, this study provides evidence that the strength of sharing 2 alleles can be used as a reference in that siblings have high kinship relations. Based on the results of this study, STR loci D5S818, CSF1PO, and F13 are recommended when typing male-male siblings whereas STR locus FES is recommended for female-female siblings of the Madurese population. The recommended main STR loci in male-female siblings of Madurese ethnicity are D13S317, D8S1179, FES, and CSF1PO.