Elderly population will continue to grow. Wrinkles are one of signs of skin aging because of the progressive loss of the dermal matrix content. Collagen is the most abundant dermal protein that it's content per unit area of skin surface decreases approximately 1% per year. The science and technology of modern engineering for wrinkle improvement has continued to evolve. Skin rejuvenation techniques for wrinkle repair have changed dramatically with the development of lasers. This study aimed to evaluate rejuvenation of wrinkle repair through collagen induction using a novel picosecond laser. Twenty subjects between 36 and 55 years old with wrinkle in the forearm were recruited. Before and 4 weeks after the subject was given treatment, a clinical examination and biopsy for histopathological examination was carried out as a pre and post-test evaluation, then treatment was carried out using a picosecond laser. Patients were predominantly female with a mean age of 42.5. At 1 month follow-up, a significant improvement was noted in wrinkle and collagen density (p<0.05). This picosecond laser was found to be effective in treating wrinkle through collagen induction which was confirmed by clinical and collagen density examination. It is believed that the high energy delivered by the picosecond laser is absorbed by intraepidermal melanin to form laser induced optical breakdown that converted into pressure waves that propagate into the dermis. This barotrauma may lead to changes in the dermis that result in collagen induction and dermal improvement. Picosecond laser is an effective option for rejuvenation through collagen induction.