TY - JOUR
T1 - Regeneration of salivary gland defects of diabetic wistar rats post human dental pulp stem cells intraglandular transplantation on acinar cell vacuolization and interleukin-10 serum level
AU - Narmada, Ida Bagus
AU - Laksono, Valerian
AU - Nugraha, Alexander Patera
AU - Ernawati, Diah Savitri
AU - Winias, Saka
AU - Prahasanti, Chiquita
AU - Dinaryanti, Aristika
AU - Susilowati, Helen
AU - Hendrianto, Eryk
AU - Ihsan, Igo Syaiful
AU - Rantam, Fedik Abdul
N1 - Funding Information:
Penelitian Dasar Unggulan Perguruan Tinggi (PDUPT) of the Ministry of Research, Technology and Higher Education of the Republic of Indonesia (Kemenristekdikti RI) (Grant No. 893/UN3/2018). Acknowledgement: The authors would like to thank the Doctral of Medical Science, Faculty of Medicine, Faculty of Dental Medicine, Stem Cell Research and Development Centre, Universitas Airlangga for its support.
Funding Information:
Authors’ Contributions: IBN, VL, APN, DSE, SW, CP, FAR designed the study, performed the data collection, data analysis and interpretation, wrote the manuscript and reviewed the manuscript. AD, HS, EH, ISI performed the data collection, data analysis and interpretation, and wrote the manuscript. All authors declare that they contributed to critical review of intellectual content and approval of the final version to be published. Financial Support: Penelitian Dasar Unggulan Perguruan Tinggi (PDUPT) of the Ministry of Research, Technology and Higher Education of the Republic of Indonesia (Kemenristekdikti RI) (Grant No. 893/UN3/2018). Conflict of Interest: The authors declare no conflicts of interest. Acknowledgement: The authors would like to thank the Doctral of Medical Science, Faculty of Medicine, Faculty of Dental Medicine, Stem Cell Research and Development Centre, Universitas Airlangga for its support.
Publisher Copyright:
© 2019, Association of Support to Oral Health Research (APESB). All rights reserved.
PY - 2019
Y1 - 2019
N2 - Objective: To investigate the regeneration of rat’s salivary gland diabetic defect after intraglandular transplantation of Human Dental Pulp Stem Cells (HDPSCs) on acinar cell vacuolization and Interleukin-10 (IL-10). Material and Methods: HDPSCs isolated from the dental pulp of first premolars #34. HDPSCs from the 3rd passage was characterized by immunocytochemistry of CD73, CD90, CD105 and CD45. Twenty-four male Wistar rats, 3-month-old, 250-300 grams induced with Streptozotocin 30 mg/kg body weight to create diabetes mellitus (DM) divided into 4 groups (n=6); positive control group on Day-7; positive control group on Day-14; treatment group Day-7 (DM+5.105HDPSCs); treatment group on Day-14. On Day-7 and Day-14, rats were sacrificed. Histopathological examination performed to analyze acinar cells vacuolization while Enzyme-linked Immunoabsorbent Assay to measure IL-10 serum level. Data obtained were analyzed statistically using multiple comparisons Bonferroni test, Kruskal Wallis, Shapiro-Wilk and Levene’s test result. Results: The highest acinar cell vacuolization found in control group Day 14 (0.239 ± 0.132), meanwhile the lowest acinar cell vacuolization found in treatment group Day 7 (0.019 ± 0.035) with significant difference (p=0.003). The highest IL-10 serum level found in treatment group Day 14 (175.583 ± 120.075) with significant difference (p=0.001). Conclusion: Transplantation of HDPSC was able to regenerate submandibular salivary gland defects in diabetic rats by decreasing acinar cell vacuolization and slightly increase IL-10 serum level.
AB - Objective: To investigate the regeneration of rat’s salivary gland diabetic defect after intraglandular transplantation of Human Dental Pulp Stem Cells (HDPSCs) on acinar cell vacuolization and Interleukin-10 (IL-10). Material and Methods: HDPSCs isolated from the dental pulp of first premolars #34. HDPSCs from the 3rd passage was characterized by immunocytochemistry of CD73, CD90, CD105 and CD45. Twenty-four male Wistar rats, 3-month-old, 250-300 grams induced with Streptozotocin 30 mg/kg body weight to create diabetes mellitus (DM) divided into 4 groups (n=6); positive control group on Day-7; positive control group on Day-14; treatment group Day-7 (DM+5.105HDPSCs); treatment group on Day-14. On Day-7 and Day-14, rats were sacrificed. Histopathological examination performed to analyze acinar cells vacuolization while Enzyme-linked Immunoabsorbent Assay to measure IL-10 serum level. Data obtained were analyzed statistically using multiple comparisons Bonferroni test, Kruskal Wallis, Shapiro-Wilk and Levene’s test result. Results: The highest acinar cell vacuolization found in control group Day 14 (0.239 ± 0.132), meanwhile the lowest acinar cell vacuolization found in treatment group Day 7 (0.019 ± 0.035) with significant difference (p=0.003). The highest IL-10 serum level found in treatment group Day 14 (175.583 ± 120.075) with significant difference (p=0.001). Conclusion: Transplantation of HDPSC was able to regenerate submandibular salivary gland defects in diabetic rats by decreasing acinar cell vacuolization and slightly increase IL-10 serum level.
KW - Acinar Cells
KW - Diabetes Mellitus
KW - Interleukin-10
KW - Totipotent Stem Cells
UR - http://www.scopus.com/inward/record.url?scp=85073566796&partnerID=8YFLogxK
U2 - 10.4034/PBOCI.2019.191.144
DO - 10.4034/PBOCI.2019.191.144
M3 - Article
AN - SCOPUS:85073566796
SN - 1519-0501
VL - 19
JO - Pesquisa Brasileira em Odontopediatria e Clinica Integrada
JF - Pesquisa Brasileira em Odontopediatria e Clinica Integrada
IS - 1
M1 - e5002
ER -
