TY - JOUR
T1 - Recovery and partial purification of thermophilic β-xylosidase derived from recombinant Bacillus megaterium MS941 by aqueous two-phase system
AU - Sofijan, Hadi
AU - Shun, Tan Joo
AU - Abbasiliasi, Sahar
AU - Mustafa, Shuhaimi
AU - Puspaningsih, Nyoman Tri
AU - Kadkhodaei, Saeid
AU - Ariff, Arbakariya B.
N1 - Publisher Copyright:
© 2017 Taylor & Francis.
PY - 2017/3/24
Y1 - 2017/3/24
N2 - A polymer–salt-based aqueous two-phase system (ATPS) was developed for the effective extraction and purification of extracellular β-xylosidase from the fermentation broth of recombinant Bacillus megaterium MS941. The effect of molecular weight (MW) of polyethylene glycol (PEG), tie-line length (TLL), volume ratio (VR), crude loading and pH on the recovery performance was evaluated. Under the optimal extraction conditions, β-xylosidase was successfully purified up to 23-fold with a recovery yield of 99% in the bottom salt-rich phase at PEG 4,000/potassium phosphate ATPS comprising TLL of 41.8, VR of 2.3, crude loading (CL) of 30% (w/w) at pH 6.
AB - A polymer–salt-based aqueous two-phase system (ATPS) was developed for the effective extraction and purification of extracellular β-xylosidase from the fermentation broth of recombinant Bacillus megaterium MS941. The effect of molecular weight (MW) of polyethylene glycol (PEG), tie-line length (TLL), volume ratio (VR), crude loading and pH on the recovery performance was evaluated. Under the optimal extraction conditions, β-xylosidase was successfully purified up to 23-fold with a recovery yield of 99% in the bottom salt-rich phase at PEG 4,000/potassium phosphate ATPS comprising TLL of 41.8, VR of 2.3, crude loading (CL) of 30% (w/w) at pH 6.
KW - Aqueous two-phase system
KW - polyethylene glycol
KW - purification
KW - β-xylosidase
UR - http://www.scopus.com/inward/record.url?scp=85009266954&partnerID=8YFLogxK
U2 - 10.1080/01496395.2016.1268159
DO - 10.1080/01496395.2016.1268159
M3 - Article
AN - SCOPUS:85009266954
SN - 0149-6395
VL - 52
SP - 834
EP - 842
JO - Separation Science and Technology
JF - Separation Science and Technology
IS - 5
ER -