Recovery and partial purification of thermophilic β-xylosidase derived from recombinant Bacillus megaterium MS941 by aqueous two-phase system

Hadi Sofijan, Tan Joo Shun, Sahar Abbasiliasi, Shuhaimi Mustafa, Nyoman Tri Puspaningsih, Saeid Kadkhodaei, Arbakariya B. Ariff

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

A polymer–salt-based aqueous two-phase system (ATPS) was developed for the effective extraction and purification of extracellular β-xylosidase from the fermentation broth of recombinant Bacillus megaterium MS941. The effect of molecular weight (MW) of polyethylene glycol (PEG), tie-line length (TLL), volume ratio (VR), crude loading and pH on the recovery performance was evaluated. Under the optimal extraction conditions, β-xylosidase was successfully purified up to 23-fold with a recovery yield of 99% in the bottom salt-rich phase at PEG 4,000/potassium phosphate ATPS comprising TLL of 41.8, VR of 2.3, crude loading (CL) of 30% (w/w) at pH 6.

Original languageEnglish
Pages (from-to)834-842
Number of pages9
JournalSeparation Science and Technology
Volume52
Issue number5
DOIs
Publication statusPublished - 24 Mar 2017

Keywords

  • Aqueous two-phase system
  • polyethylene glycol
  • purification
  • β-xylosidase

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