TY - GEN
T1 - Ramiprilat Effects on Endothelial Progenitor Cells Migration is Increased by Human Umbilical Cord Blood-Mesenchymal Stem Cells derived Secretome
AU - Her Oktaviono, Yudi
AU - Alfia Isaridha, Ilma
AU - Sandra, Ferry
AU - Lefi, Achmad
AU - Subagjo, Agus
N1 - Publisher Copyright:
© 2020 ACM.
PY - 2020/11/6
Y1 - 2020/11/6
N2 - Endothelial progenitor cells (EPCs) have a critical role in angiogenesis and vasculogenesis of coronary artery disease (CAD) patients. Secretome of human Umbilical Cord Blood-Mesenchymal Stem Cell (hUCB-MSCs) can promote neovascularization. Ramiprilat is an active metabolite of ramipril that has shown benefit in cardiovascular disease. The effect of hUCB-MSCs-derived secretome alone or combination with ramiprilat on EPCs migration is not yet elucidated. This study aimed to identify the effect of hUCB- MSC derived secretome and its combination with ramiprilat on EPCs migration. EPCs were collected from peripheral blood of CAD patient and cultured in the Stemline II medium. Cultured EPCs were then divided into groups of control, ramiprilat 10 μmol, hUCB-MSCs derived secretome (2%, 10%, and 20%), and its combination. The migration of EPCs was assessed using a Boyden chamber assay. Ramiprilat and hUCB-MSCs-derived secretome at all doses increase EPCs migration in dose-dependent manner. Combination of hUCB-MSCs-derived secretome at dose 10% and 20% and ramiprilat significantly increase migrated cells compared to ramiprilat only and secretome only group (p<0.001). In conclusion, hUCB-MSCs-derived secretome and ramiprilat enhance EPCs migration and combination of those two substances furtherly increased the migrated cells. hUCB-MSCs-derived secretome has the potential as regenerative treatment for CAD patients.
AB - Endothelial progenitor cells (EPCs) have a critical role in angiogenesis and vasculogenesis of coronary artery disease (CAD) patients. Secretome of human Umbilical Cord Blood-Mesenchymal Stem Cell (hUCB-MSCs) can promote neovascularization. Ramiprilat is an active metabolite of ramipril that has shown benefit in cardiovascular disease. The effect of hUCB-MSCs-derived secretome alone or combination with ramiprilat on EPCs migration is not yet elucidated. This study aimed to identify the effect of hUCB- MSC derived secretome and its combination with ramiprilat on EPCs migration. EPCs were collected from peripheral blood of CAD patient and cultured in the Stemline II medium. Cultured EPCs were then divided into groups of control, ramiprilat 10 μmol, hUCB-MSCs derived secretome (2%, 10%, and 20%), and its combination. The migration of EPCs was assessed using a Boyden chamber assay. Ramiprilat and hUCB-MSCs-derived secretome at all doses increase EPCs migration in dose-dependent manner. Combination of hUCB-MSCs-derived secretome at dose 10% and 20% and ramiprilat significantly increase migrated cells compared to ramiprilat only and secretome only group (p<0.001). In conclusion, hUCB-MSCs-derived secretome and ramiprilat enhance EPCs migration and combination of those two substances furtherly increased the migrated cells. hUCB-MSCs-derived secretome has the potential as regenerative treatment for CAD patients.
KW - endothelial progenitor cells
KW - human umbilical cord blood
KW - mesenchymal stem cells
KW - migration
KW - ramiprilat
KW - secretome
UR - http://www.scopus.com/inward/record.url?scp=85103829217&partnerID=8YFLogxK
U2 - 10.1145/3444884.3444914
DO - 10.1145/3444884.3444914
M3 - Conference contribution
AN - SCOPUS:85103829217
T3 - ACM International Conference Proceeding Series
SP - 154
EP - 159
BT - Proceedings of 2020 7th International Conference on Biomedical and Bioinformatics Engineering, ICBBE 2020
PB - Association for Computing Machinery
T2 - 7th International Conference on Biomedical and Bioinformatics Engineering, ICBBE 2020
Y2 - 6 November 2020 through 9 November 2020
ER -
