TY - JOUR
T1 - Radioadaptation response of parotid salivary glands acinar cells induced the low dose of X-ray radiation from skull radiography and then challenged the therapy dose of gamma-ray radiation
T2 - The measurements of Hsp70 expression, SOD2 activation, and MDA concentration
AU - Supriyadi,
AU - Prija, Trijono Karmawan Sukana
AU - Rahayu, Retno Pudji
N1 - Publisher Copyright:
© 2018 University of Dicle.
PY - 2018
Y1 - 2018
N2 - The aim of this study was to measure the Hsp70 expression, SOD2 activity and MDA concentration on radioadaptation response of parotid saliva glands acinar cells induced the low-dose of X-ray radiation from Skull Radiography. This research involved 24 males Rattus Norvegicus, wistar strains divided into 4 groups: normal control, positive control, adaptation of A (single of X-ray exposure from Skull Radiography), and an adaptation of B (chronic of X-ray exposure from Skull Radiography). The Challenge radiation exposure was performed 5 hours after adaptive radiation. The radiation was directed at the dorsal of animal's head. The animal research to be sacrificed 24 hours after challenge radiation exposure, and soon the parotid tissue was taken. Furthermore, tissue processing was performed for histopathological specimens. Hsp70 expression, SOD2 activity, and MDA concentration were measured using immunohistochemical techniques. The data were analyzed by one way ANOVA test (α = 0,05) using SPPS software version 21. The results showed that there were significant differences for Hsp70 expression, SOD2 activity and MDA concentration of parotid salivary glands acinar cells among 4 groups. The low-dose of X-ray radiation from Skull Radiography may induce radioadaptation response in acinar cells of parotid salivary glands.
AB - The aim of this study was to measure the Hsp70 expression, SOD2 activity and MDA concentration on radioadaptation response of parotid saliva glands acinar cells induced the low-dose of X-ray radiation from Skull Radiography. This research involved 24 males Rattus Norvegicus, wistar strains divided into 4 groups: normal control, positive control, adaptation of A (single of X-ray exposure from Skull Radiography), and an adaptation of B (chronic of X-ray exposure from Skull Radiography). The Challenge radiation exposure was performed 5 hours after adaptive radiation. The radiation was directed at the dorsal of animal's head. The animal research to be sacrificed 24 hours after challenge radiation exposure, and soon the parotid tissue was taken. Furthermore, tissue processing was performed for histopathological specimens. Hsp70 expression, SOD2 activity, and MDA concentration were measured using immunohistochemical techniques. The data were analyzed by one way ANOVA test (α = 0,05) using SPPS software version 21. The results showed that there were significant differences for Hsp70 expression, SOD2 activity and MDA concentration of parotid salivary glands acinar cells among 4 groups. The low-dose of X-ray radiation from Skull Radiography may induce radioadaptation response in acinar cells of parotid salivary glands.
KW - Hsp70
KW - Low dose radiation
KW - MDA
KW - Parotid gland acinar cells
KW - Radioadaptation response
KW - SOD2
KW - Skull radiography
UR - http://www.scopus.com/inward/record.url?scp=85066996331&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85066996331
SN - 1309-100X
VL - 11
SP - 804
EP - 809
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
IS - 3
ER -