Rabbit skin-derived mesenchymal stem cells (SMSCS) characterization and differentiation into osteogenic lineage

Nora Ertanti, Deya Karsari, Aristika Dinaryanti, Igo Syaiful Ihsan, Eryk Hendrianto, Rafizka Putri Iriandani, Fedik Abdul Rantam, Purwati

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Rabbits (Oryctolagus cuniculus) are widely used as experimental models for both human and veterinary medicine because of their ease to work with and relatively economic maintenance. Rabbits are useful for multiple applications in biotechnology and translational medicine from basic research to preclinical studies. Skin has been considered a potential source of adult stem cells. Skin-Derived Mesenchymal Stem Cells (SMSCs) are an abundant pluripotent, multipotent cell source and capable of undergoing in vitro osteogenic differentiation. The differentiation of SMSCs in vitro is induced by the presence of dexamethasone, ascorbic acid and β-glycerol phosphate. The characterization of Skin-Derived Mesenchymal Stem Cells (SMSCs) showed the expression of CD105, CD73, CD90 for positive markers, as well as CD45 for negative markers using immunofluorescence methods for mesenchymal stem cells (MSCs). The present work first determined the osteogenic differentiation from SMSCs for 21 days, then confirmed the characterized expression of osteocalcin, runt-related transcription factor 2 (Runx2), SRY-related HMG-box (Sox9) with immunofluorescence and analyzed by alizarin red staining for osteogenic differentiation.

Original languageEnglish
Pages (from-to)4783-4789
Number of pages7
JournalBiochemical and Cellular Archives
Publication statusPublished - 1 Dec 2019


  • Alkaline phosphatase
  • Mesenchymal stem cell
  • Osteoblast
  • Osteocalcin
  • Osteogenesis
  • Osteogenic differentiation
  • Runx2
  • SMSCs
  • Skin derived mesenchymal stem cells
  • Sox9


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