TY - JOUR
T1 - Rabbit skin-derived mesenchymal stem cells (SMSCS) characterization and differentiation into osteogenic lineage
AU - Ertanti, Nora
AU - Karsari, Deya
AU - Dinaryanti, Aristika
AU - Ihsan, Igo Syaiful
AU - Hendrianto, Eryk
AU - Iriandani, Rafizka Putri
AU - Rantam, Fedik Abdul
AU - Purwati,
N1 - Publisher Copyright:
© 2019 Connect Journal.
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Rabbits (Oryctolagus cuniculus) are widely used as experimental models for both human and veterinary medicine because of their ease to work with and relatively economic maintenance. Rabbits are useful for multiple applications in biotechnology and translational medicine from basic research to preclinical studies. Skin has been considered a potential source of adult stem cells. Skin-Derived Mesenchymal Stem Cells (SMSCs) are an abundant pluripotent, multipotent cell source and capable of undergoing in vitro osteogenic differentiation. The differentiation of SMSCs in vitro is induced by the presence of dexamethasone, ascorbic acid and β-glycerol phosphate. The characterization of Skin-Derived Mesenchymal Stem Cells (SMSCs) showed the expression of CD105, CD73, CD90 for positive markers, as well as CD45 for negative markers using immunofluorescence methods for mesenchymal stem cells (MSCs). The present work first determined the osteogenic differentiation from SMSCs for 21 days, then confirmed the characterized expression of osteocalcin, runt-related transcription factor 2 (Runx2), SRY-related HMG-box (Sox9) with immunofluorescence and analyzed by alizarin red staining for osteogenic differentiation.
AB - Rabbits (Oryctolagus cuniculus) are widely used as experimental models for both human and veterinary medicine because of their ease to work with and relatively economic maintenance. Rabbits are useful for multiple applications in biotechnology and translational medicine from basic research to preclinical studies. Skin has been considered a potential source of adult stem cells. Skin-Derived Mesenchymal Stem Cells (SMSCs) are an abundant pluripotent, multipotent cell source and capable of undergoing in vitro osteogenic differentiation. The differentiation of SMSCs in vitro is induced by the presence of dexamethasone, ascorbic acid and β-glycerol phosphate. The characterization of Skin-Derived Mesenchymal Stem Cells (SMSCs) showed the expression of CD105, CD73, CD90 for positive markers, as well as CD45 for negative markers using immunofluorescence methods for mesenchymal stem cells (MSCs). The present work first determined the osteogenic differentiation from SMSCs for 21 days, then confirmed the characterized expression of osteocalcin, runt-related transcription factor 2 (Runx2), SRY-related HMG-box (Sox9) with immunofluorescence and analyzed by alizarin red staining for osteogenic differentiation.
KW - Alkaline phosphatase
KW - Mesenchymal stem cell
KW - Osteoblast
KW - Osteocalcin
KW - Osteogenesis
KW - Osteogenic differentiation
KW - Runx2
KW - SMSCs
KW - Skin derived mesenchymal stem cells
KW - Sox9
UR - http://www.scopus.com/inward/record.url?scp=85079377993&partnerID=8YFLogxK
U2 - 10.35124/bca.2019.19.S2.4783
DO - 10.35124/bca.2019.19.S2.4783
M3 - Article
AN - SCOPUS:85079377993
SN - 0972-5075
SP - 4783
EP - 4789
JO - Biochemical and Cellular Archives
JF - Biochemical and Cellular Archives
ER -