Quantitative analysis of Quercus infectoria gall extract from supercritical fluid extraction CO₂using LC-MS/MS

Using liquid chromatography-mass spectrometry (LC-MS/MS), the phenolic content of the secondary metabolic extract of Quercus infectoria gall (Q. infectoria gall) extracted with supercritical CO2 and co-solvent methanol can be evaluated directly. 10 g of Q. infectoria gall was extracted using the SCFE CO2 technique at 50 °C under 20 MPa of pressure for 60 minutes with 1% methanol by weight. The extract contains seven bioactive components, including tannic acid (67.18%), quinic acid (7.99%), gallic acid (2.57%),maleic acid (0.02%), ellagic acid (0.22%), salicylic acid (0.03%), chlorogenic acid (0.09%), benzoic acid (0.01%), caffeic acid (0.02%), and aconitic acid (0.02%). Other compositions are rather difficult to detect due to the reproducibility of the acquired peaks.