Purification of a lethal toxin of Edwardsiella tarda

Hari Suprapto; Takaaki Hara; Toshihiro Nakai; Kiyokuni Muroga

doi:10.3147/jsfp.31.203

Purification of a lethal toxin of Edwardsiella tarda

Hari Suprapto, Takaaki Hara, Toshihiro Nakai, Kiyokuni Muroga

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

A toxin lethal to fish was purified from extracellular products (ECP) of Edwardsiella tarda by ion exchange chromatography and gel filtration. The purified toxin is a protein having proteolytic activities and lethal toxicity to Japanese eel Anguilla japonica with the LD₅₀ of 1.6 μg per g of fish body weight. Molecular weight of the protein was estimated to be 37 kDa by SDS-PAGE. SDS-PAGE, Western blot and immunodiffusion analyses revealed that the 37 kDa protein was present in the ECP and ICC (intracellular components) of virulent strains of E. tarda but not in those of avirulent strains, indicating that this toxin plays an important role in the pathogenicity of E. tarda.

Original language	English
Pages (from-to)	203-207
Number of pages	5
Journal	Fish Pathology
Volume	31
Issue number	4
DOIs	https://doi.org/10.3147/jsfp.31.203
Publication status	Published - Dec 1996
Externally published	Yes

Keywords

ECP
Edwardsiella tarda
Extracellular product
Intracellular component
Japanese eel
Toxin

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10.3147/jsfp.31.203

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title = "Purification of a lethal toxin of Edwardsiella tarda",

abstract = "A toxin lethal to fish was purified from extracellular products (ECP) of Edwardsiella tarda by ion exchange chromatography and gel filtration. The purified toxin is a protein having proteolytic activities and lethal toxicity to Japanese eel Anguilla japonica with the LD50 of 1.6 μg per g of fish body weight. Molecular weight of the protein was estimated to be 37 kDa by SDS-PAGE. SDS-PAGE, Western blot and immunodiffusion analyses revealed that the 37 kDa protein was present in the ECP and ICC (intracellular components) of virulent strains of E. tarda but not in those of avirulent strains, indicating that this toxin plays an important role in the pathogenicity of E. tarda.",

keywords = "ECP, Edwardsiella tarda, Extracellular product, Intracellular component, Japanese eel, Toxin",

author = "Hari Suprapto and Takaaki Hara and Toshihiro Nakai and Kiyokuni Muroga",

year = "1996",

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T1 - Purification of a lethal toxin of Edwardsiella tarda

AU - Suprapto, Hari

AU - Hara, Takaaki

AU - Nakai, Toshihiro

AU - Muroga, Kiyokuni

PY - 1996/12

Y1 - 1996/12

N2 - A toxin lethal to fish was purified from extracellular products (ECP) of Edwardsiella tarda by ion exchange chromatography and gel filtration. The purified toxin is a protein having proteolytic activities and lethal toxicity to Japanese eel Anguilla japonica with the LD50 of 1.6 μg per g of fish body weight. Molecular weight of the protein was estimated to be 37 kDa by SDS-PAGE. SDS-PAGE, Western blot and immunodiffusion analyses revealed that the 37 kDa protein was present in the ECP and ICC (intracellular components) of virulent strains of E. tarda but not in those of avirulent strains, indicating that this toxin plays an important role in the pathogenicity of E. tarda.

AB - A toxin lethal to fish was purified from extracellular products (ECP) of Edwardsiella tarda by ion exchange chromatography and gel filtration. The purified toxin is a protein having proteolytic activities and lethal toxicity to Japanese eel Anguilla japonica with the LD50 of 1.6 μg per g of fish body weight. Molecular weight of the protein was estimated to be 37 kDa by SDS-PAGE. SDS-PAGE, Western blot and immunodiffusion analyses revealed that the 37 kDa protein was present in the ECP and ICC (intracellular components) of virulent strains of E. tarda but not in those of avirulent strains, indicating that this toxin plays an important role in the pathogenicity of E. tarda.

KW - ECP

KW - Edwardsiella tarda

KW - Extracellular product

KW - Intracellular component

KW - Japanese eel

KW - Toxin

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DO - 10.3147/jsfp.31.203

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SN - 0388-788X

VL - 31

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EP - 207

JO - Fish Pathology

JF - Fish Pathology

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ER -

Purification of a lethal toxin of Edwardsiella tarda

Abstract

Keywords

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