Purification of a lethal toxin of Edwardsiella tarda

Hari Suprapto, Takaaki Hara, Toshihiro Nakai, Kiyokuni Muroga

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21 Citations (Scopus)


A toxin lethal to fish was purified from extracellular products (ECP) of Edwardsiella tarda by ion exchange chromatography and gel filtration. The purified toxin is a protein having proteolytic activities and lethal toxicity to Japanese eel Anguilla japonica with the LD50 of 1.6 μg per g of fish body weight. Molecular weight of the protein was estimated to be 37 kDa by SDS-PAGE. SDS-PAGE, Western blot and immunodiffusion analyses revealed that the 37 kDa protein was present in the ECP and ICC (intracellular components) of virulent strains of E. tarda but not in those of avirulent strains, indicating that this toxin plays an important role in the pathogenicity of E. tarda.

Original languageEnglish
Pages (from-to)203-207
Number of pages5
JournalFish Pathology
Issue number4
Publication statusPublished - Dec 1996
Externally publishedYes


  • ECP
  • Edwardsiella tarda
  • Extracellular product
  • Intracellular component
  • Japanese eel
  • Toxin


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