TY - JOUR
T1 - Purification, crystallization and preliminary X-ray analysis of a thermostable glycoside hydrolase family 43 β-xylosidase from Geobacillus thermoleovorans IT-08
AU - Rohman, Ali
AU - Van Oosterwijk, Niels
AU - Kralj, Slavko
AU - Dijkhuizen, Lubbert
AU - Dijkstra, Bauke W.
AU - Puspaningsih, Ni Nyoman Tri
PY - 2007
Y1 - 2007
N2 - The main enzymes involved in xylan-backbone hydrolysis are endo-1,4-β-xylanase and β-xylosidase. β-Xylosidase converts the xylo-oligosaccharides produced by endo-1,4-β-xylanase into xylose monomers. The β-xylosidase from the thermophilic Geobacillus thermoleovorans IT-08, a member of glycoside hydrolase family 43, was crystallized at room temperature using the hanging-drop vapour-diffusion method. Two crystal forms were observed. Bipyramid-shaped crystals belonging to space group P432 12, with unit-cell parameters a = b = 62.53, c = 277.4 Å diffracted to 1.55 Å resolution. The rectangular crystals belonged to space group P21, with unit-cell parameters a = 57.94, b = 142.1, c = 153.9 Å, β = 90.5°, and diffracted to 1.80 Å resolution.
AB - The main enzymes involved in xylan-backbone hydrolysis are endo-1,4-β-xylanase and β-xylosidase. β-Xylosidase converts the xylo-oligosaccharides produced by endo-1,4-β-xylanase into xylose monomers. The β-xylosidase from the thermophilic Geobacillus thermoleovorans IT-08, a member of glycoside hydrolase family 43, was crystallized at room temperature using the hanging-drop vapour-diffusion method. Two crystal forms were observed. Bipyramid-shaped crystals belonging to space group P432 12, with unit-cell parameters a = b = 62.53, c = 277.4 Å diffracted to 1.55 Å resolution. The rectangular crystals belonged to space group P21, with unit-cell parameters a = 57.94, b = 142.1, c = 153.9 Å, β = 90.5°, and diffracted to 1.80 Å resolution.
KW - Geobacillus thermoleovorans IT-08
KW - Glycoside hydrolase family 43
KW - β-xylosidase
UR - http://www.scopus.com/inward/record.url?scp=36249012450&partnerID=8YFLogxK
U2 - 10.1107/S1744309107046015
DO - 10.1107/S1744309107046015
M3 - Article
C2 - 18007043
AN - SCOPUS:36249012450
SN - 1744-3091
VL - 63
SP - 932
EP - 935
JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
IS - 11
ER -