TY - JOUR
T1 - Pulp fibroblast cell apoptosis after application of hema dentine bonding material with ethano land water solvent
AU - Nandarani, Ratih Elisa
AU - Widjiastuti, Ira
AU - Mooduto, Latief
N1 - Publisher Copyright:
© 2019, Associacao Brasileira de Divulgacao Cientifica. All rights reserved.
PY - 2019/5/1
Y1 - 2019/5/1
N2 - The most common main materials for dentin bonding for composite resin restoration is 2-hydroxyethyl methacrylate (HEMA). HEMA has beneficial physical and chemical properties, and stable, yet toxic. The addition of ethanol or water, may reduce the toxic effect of HEMA. Ethanol solvent has lower H-bonding capacity compared to water solvent, so it can bind less free radicals from the residual monomer. This study aimed to analyze apoptosis due to dentine bonding application with ethanol and water solvent. Fibroblast culture cells were obtained from extracted third molar, by means of tripsinasion method. The cells were divided into 4 groups as reached confluent: cell culture without treatment as control, cell culture with scaffold chitosan, cell culture with scaffold and polymerized dentin bonding with ethanol or water solvent. Apoptosis observation was conducted using immunohistochemistry method with ethidium bromide acridin orange staining, under fluorescent microscope with 40× magnification. There was a significant difference among groups (p=0.0001), yet no differences found between different solvent. Apoptosis rate in fibroblast cells culture exposed to HEMA bonding with ethanol solvent was 67%, while the cells exposed to HEMA bonding with water solvent was 44%. The effect of dentin bonding with ethanol solvent and water solvent towards apoptosis rate of pulp fibroblast cells is not different.
AB - The most common main materials for dentin bonding for composite resin restoration is 2-hydroxyethyl methacrylate (HEMA). HEMA has beneficial physical and chemical properties, and stable, yet toxic. The addition of ethanol or water, may reduce the toxic effect of HEMA. Ethanol solvent has lower H-bonding capacity compared to water solvent, so it can bind less free radicals from the residual monomer. This study aimed to analyze apoptosis due to dentine bonding application with ethanol and water solvent. Fibroblast culture cells were obtained from extracted third molar, by means of tripsinasion method. The cells were divided into 4 groups as reached confluent: cell culture without treatment as control, cell culture with scaffold chitosan, cell culture with scaffold and polymerized dentin bonding with ethanol or water solvent. Apoptosis observation was conducted using immunohistochemistry method with ethidium bromide acridin orange staining, under fluorescent microscope with 40× magnification. There was a significant difference among groups (p=0.0001), yet no differences found between different solvent. Apoptosis rate in fibroblast cells culture exposed to HEMA bonding with ethanol solvent was 67%, while the cells exposed to HEMA bonding with water solvent was 44%. The effect of dentin bonding with ethanol solvent and water solvent towards apoptosis rate of pulp fibroblast cells is not different.
KW - 2-hydroxyethyl methacrylate
KW - Apoptosis
KW - Dentine bonding
KW - Ethanol solvent
KW - Water solvent
UR - http://www.scopus.com/inward/record.url?scp=85067487271&partnerID=8YFLogxK
U2 - 10.1590/0103-6440201902524
DO - 10.1590/0103-6440201902524
M3 - Article
C2 - 31166391
AN - SCOPUS:85067487271
SN - 0103-6440
VL - 30
SP - 208
EP - 212
JO - Brazilian Dental Journal
JF - Brazilian Dental Journal
IS - 3
ER -