Stroke is a serious and life-threatening medical condition that occurs when the blood that supplies to part of the brain is cut off. It also leads to main disability. However, the post-stroke rehabilitation today seems to lack in giving a significant recovery. The post-stroke rehabilitation requires more interaction during movements to improve the motor skill. It is achievable by developing robotics rehabilitation that employs proportional myoelectric control method which utilizes muscle signal to control the actuator, resulting in real-time movement comparable to myoelectric signal value. Therefore, this study was intended to investigate the robotics rehabilitation movement response using the prototype therapeutics device towards the arm muscle signal of the healthy subjects and the subjects of post-stroke. The study was conducted by utilizing the signal voltage value of muscle bicep brachii using electromyograph (EMG) and NI myDAQ. The signal was programmed by NI LabVIEW software. It produced pulse width modulation (PWM) value to move the actuator in the servo motor. The result showed that the regression value of healthy subject's movement response was linear for angle 30° - 120°. The myoelectric control method gave comparable movement value between the angle of the arm and post-stroke therapy device prototype.