TY - JOUR
T1 - PROTEIN INTERACTION STUDIES OF CROSSLINKED ENDOLEVANASE AGGREGATES FROM BACILLUS LEHENSIS G1
AU - Makki, Hotaf Hassan
AU - Jaafar, Nardiah Rizwana
AU - Rahman, Roshanida A.
AU - Rahmat, Zaidah
AU - Puspaningsih, Ni Nyoman Tri
AU - Illias, Rosli Md
N1 - Publisher Copyright:
© 2024 Penerbit UTM Press. All rights reserved.
PY - 2024/3
Y1 - 2024/3
N2 - The efficiency of cross-linked enzyme aggregates (CLEAs) is mainly affected by the strength and binding site of the formed linkages between the enzyme and cross-linker. Therefore, this study investigated the impact of different macromolecular cross-linkers on various functional groups, their binding energy, and intermolecular interaction in generating CLEAs of endolevanase from Bacillus lehensis G1 (rlevblg1), through the combination of computational and experimental analysis. Due to the distanced bonding of dextran from the active site, rlevblg1 cross-linked with dextran (rlevblg1-dexCLEAs) exhibited the highest binding affinity (− 7.1 kcal/mol) and activity recovery compared to six other cross-linkers. Thus, the role of computational cross-linker screening is confirmed as a crucial step to predict strong attachment and construct efficient CLEAs.
AB - The efficiency of cross-linked enzyme aggregates (CLEAs) is mainly affected by the strength and binding site of the formed linkages between the enzyme and cross-linker. Therefore, this study investigated the impact of different macromolecular cross-linkers on various functional groups, their binding energy, and intermolecular interaction in generating CLEAs of endolevanase from Bacillus lehensis G1 (rlevblg1), through the combination of computational and experimental analysis. Due to the distanced bonding of dextran from the active site, rlevblg1 cross-linked with dextran (rlevblg1-dexCLEAs) exhibited the highest binding affinity (− 7.1 kcal/mol) and activity recovery compared to six other cross-linkers. Thus, the role of computational cross-linker screening is confirmed as a crucial step to predict strong attachment and construct efficient CLEAs.
KW - Endolevanase
KW - computational analysis
KW - cross-linked enzyme aggregates
KW - enzyme immobilization
KW - macromolecular cross-linkers
UR - http://www.scopus.com/inward/record.url?scp=85185669235&partnerID=8YFLogxK
U2 - 10.11113/jurnalteknologi.v86.20888
DO - 10.11113/jurnalteknologi.v86.20888
M3 - Article
AN - SCOPUS:85185669235
SN - 0127-9696
VL - 86
SP - 159
EP - 167
JO - Jurnal Teknologi (Sciences and Engineering)
JF - Jurnal Teknologi (Sciences and Engineering)
IS - 2
ER -