TY - JOUR
T1 - Protective effect of propolis extract in kidney male mice (Mus musculus) induced by lead acetate
AU - Henra, Citrasari
AU - Misaco, Wiwik
AU - Plumeriastuti, Hani
N1 - Funding Information:
The authors are very thankful to Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya for providing all the type of facilities to conduct this research.
Publisher Copyright:
© 2018 Journal of International Dental and Medical Research.
PY - 2017
Y1 - 2017
N2 - The aim of this research was to investigate the protective effect of propolis extract from Apis mellifera, on histopathological changes of the kidney induced by lead acetate in mice (Mus musculus). 25 male mice were randomly divided into five groups and administered orally with different treatments for 35 days. The treatment were Group C- (CMC-Na 1.5% and Tween 80 0.5% continued with aquadest one hour after the first administration), Group C+ (CMC-Na 1.5% and Tween 80 0.5% and continued with 20 mg/kg bw of lead acetate), and treatment group T1, T2, T3 (200, 400, 800 mg/kg bw propolis extract and continued with 20 mg/kg bw of lead acetate). After treatments, tissues were processed, and histopathological evaluation were examined using Arshad Scoring method. The result showed necrosis and tubular cast in the treatment group of T3 (1.44±0.09a and 1.04±0.43ab) have no significant difference with group of C- (1.24±0.33a and 0.96±026a). The mean value of necrosis and tubular cast in group T3 is the lowest, while the mean value of hydropic degeneration is the highest. It indicates the reversible cell injury. Thus, the effective dose of propolis extract used in this research is 800 mg/kg bw.
AB - The aim of this research was to investigate the protective effect of propolis extract from Apis mellifera, on histopathological changes of the kidney induced by lead acetate in mice (Mus musculus). 25 male mice were randomly divided into five groups and administered orally with different treatments for 35 days. The treatment were Group C- (CMC-Na 1.5% and Tween 80 0.5% continued with aquadest one hour after the first administration), Group C+ (CMC-Na 1.5% and Tween 80 0.5% and continued with 20 mg/kg bw of lead acetate), and treatment group T1, T2, T3 (200, 400, 800 mg/kg bw propolis extract and continued with 20 mg/kg bw of lead acetate). After treatments, tissues were processed, and histopathological evaluation were examined using Arshad Scoring method. The result showed necrosis and tubular cast in the treatment group of T3 (1.44±0.09a and 1.04±0.43ab) have no significant difference with group of C- (1.24±0.33a and 0.96±026a). The mean value of necrosis and tubular cast in group T3 is the lowest, while the mean value of hydropic degeneration is the highest. It indicates the reversible cell injury. Thus, the effective dose of propolis extract used in this research is 800 mg/kg bw.
KW - Free radicals
KW - Kidney
KW - Lead
KW - Propolis
KW - Reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=85050297591&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85050297591
SN - 1309-100X
VL - 10
SP - 1060
EP - 1065
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
IS - 3
ER -