TY - JOUR
T1 - Protection against brain histopathological damage in experimental cerebral malaria models after exposure to hyperbaric oxigent
AU - Utami, Prawesty Diah
AU - Hadi, Usman
AU - Dachlan, Yoes Prijatna
AU - Suryokusumo, Guritno
AU - Loeki Enggar Fitri, R.
AU - Yudo, Varidianto
N1 - Publisher Copyright:
© RJPT All right reserved.
PY - 2021/7
Y1 - 2021/7
N2 - In this study, brain damage caused by cerebral malaria was induced by parasitized erythrocyte rupture and sequestration, which led to inflammation and blood vessel damage. Therefore, this research objective to determine the effect of oxygen administration on the histopathological features and sequestration of CD3 lymphocyte T cells on Plasmodium berghei ANKA/PbA-infected vascular endothelial brain tissue of mice. The study samples consisted of 39 C57BL/6 mice, which were divided into 3 groups: G1 contained normal mice; G2 contained PbA-infected mice; G3 were mice infected with PbA, and administered HBO 2.4 ATA for 10 days straight. Histopathological examination of the of brain tissue and CD3 lymphocyte T cell expression was carried out using immuno-histochemical at the end of the study. Therefore, the results of this study indicate that HBO administration can reduce the level of parasites, can improve the histopathological features of the brain, and can reduce the sequestration of CD3 cells in the brain's blood vessels. According to the results, it can be concluded that 10 sessions of HBO 2.4 ATA exposure can reduce the level of parasites, enhance the histopathological features of brain tissue and decrease the sequestration of CD3 lymphocyte T cells.
AB - In this study, brain damage caused by cerebral malaria was induced by parasitized erythrocyte rupture and sequestration, which led to inflammation and blood vessel damage. Therefore, this research objective to determine the effect of oxygen administration on the histopathological features and sequestration of CD3 lymphocyte T cells on Plasmodium berghei ANKA/PbA-infected vascular endothelial brain tissue of mice. The study samples consisted of 39 C57BL/6 mice, which were divided into 3 groups: G1 contained normal mice; G2 contained PbA-infected mice; G3 were mice infected with PbA, and administered HBO 2.4 ATA for 10 days straight. Histopathological examination of the of brain tissue and CD3 lymphocyte T cell expression was carried out using immuno-histochemical at the end of the study. Therefore, the results of this study indicate that HBO administration can reduce the level of parasites, can improve the histopathological features of the brain, and can reduce the sequestration of CD3 cells in the brain's blood vessels. According to the results, it can be concluded that 10 sessions of HBO 2.4 ATA exposure can reduce the level of parasites, enhance the histopathological features of brain tissue and decrease the sequestration of CD3 lymphocyte T cells.
KW - CD3 cells
KW - Cerebral malaria
KW - Endothel
KW - Histopathological
KW - Hyperbaric oxygen
UR - http://www.scopus.com/inward/record.url?scp=85112730263&partnerID=8YFLogxK
U2 - 10.52711/0974-360X.2021.00665
DO - 10.52711/0974-360X.2021.00665
M3 - Article
AN - SCOPUS:85112730263
SN - 0974-3618
VL - 14
SP - 3833
EP - 3838
JO - Research Journal of Pharmacy and Technology
JF - Research Journal of Pharmacy and Technology
IS - 7
ER -