TY - JOUR
T1 - Protease-dependent hemagglutinin cleavage contributes to alteration in chicken hemagglutination by the H3N2 influenza a virus
AU - Yamaoka, Masaoki
AU - Makino, Akiko
AU - Sasahara, Kenji
AU - Mareta Nastri, Aldise
AU - Wilan Krisna, Luh Ade
AU - Frederika Purhito, Edith
AU - Djoko Poetranto, Emmanuel
AU - Wulandari, Laksmi
AU - Yudhawati, Resti
AU - Setiawati, Landia
AU - Asih Setyoningrum, Retno
AU - Shinya, Kyoko
PY - 2013
Y1 - 2013
N2 - The human influenza A virus (H3N2) has been the predominant influenza strain since 1992, and one property of this virus is non-agglutination of chicken erythrocytes [Ch(-) virus]. The Ch(-) virus in our study was able to acquire chicken hemagglutination [Ch(+)] by trypsin passage but not by chymotrypsin passage. Moreover, the trypsin-passaged Ch(+) viruses reacquired the Ch(-) property after a further chymotrypsin passage. In particular, genetic analysis showed no evidence of mutations in the hemagglutinin (HA) gene during either trypsin or chymotrypsin passages: the only differences found were in the HA cleavage sites between the trypsin-passaged virus and the chymotrypsin-passaged virus as determined by the N-terminal amino acid sequence. These results suggested that protease-dependent differences at the viral HA cleavage site, rather than genetic mutations, are likely to have a significant effect on the viral ability to produce chicken hemagglutination.
AB - The human influenza A virus (H3N2) has been the predominant influenza strain since 1992, and one property of this virus is non-agglutination of chicken erythrocytes [Ch(-) virus]. The Ch(-) virus in our study was able to acquire chicken hemagglutination [Ch(+)] by trypsin passage but not by chymotrypsin passage. Moreover, the trypsin-passaged Ch(+) viruses reacquired the Ch(-) property after a further chymotrypsin passage. In particular, genetic analysis showed no evidence of mutations in the hemagglutinin (HA) gene during either trypsin or chymotrypsin passages: the only differences found were in the HA cleavage sites between the trypsin-passaged virus and the chymotrypsin-passaged virus as determined by the N-terminal amino acid sequence. These results suggested that protease-dependent differences at the viral HA cleavage site, rather than genetic mutations, are likely to have a significant effect on the viral ability to produce chicken hemagglutination.
KW - H3N2 influenza A virus
KW - Hemagglutination
KW - Protease
UR - http://www.scopus.com/inward/record.url?scp=84888097200&partnerID=8YFLogxK
U2 - 10.7883/yoken.66.526
DO - 10.7883/yoken.66.526
M3 - Article
C2 - 24270143
AN - SCOPUS:84888097200
SN - 1344-6304
VL - 66
SP - 526
EP - 529
JO - Japanese Journal of Infectious Diseases
JF - Japanese Journal of Infectious Diseases
IS - 6
ER -