TY - JOUR
T1 - Propolis-enhanced Calcium Hydroxide Combination for Direct Pulp Capping
T2 - Impact on Oxidative Stress Markers in Wistar Rat Teeth
AU - Widjiastuti, Ira
AU - Kunarti, Sri
AU - Ningtyas, Evri Kusumah
AU - Atikah, Ayu Rafania
AU - Basalamah, Khadijah Fauzi
AU - Puspita, Amelia Evita
AU - Widianti, Novelia Ayu
AU - Noor, Tengku Natasha Eleena Binti Tengku Ahmad
N1 - Publisher Copyright:
© 2024, Iranian Centre for Endodontic Research. All rights reserved.
PY - 2024/3/1
Y1 - 2024/3/1
N2 - Introduction: This study explored the effects of propolis extract calcium hydroxide (Ca(OH)2 ) combination on malondialdehyde and superoxide dismutase expression in dental pulp, aiming to assess its potential as a direct pulp capping material. Materials and Methods: Thirty male Wistar rats were randomly assigned to three groups. Mandibular molar teeth were prepared using a low-speed round bur. In Group I, no material was applied; in Group II, teeth were treated with Ca(OH)2; and in Group III, teeth were treated with Propolis extract-Ca(OH)2, followed by Cention N filling. Immunohistochemistry was conducted on pulp tissue samples obtained on the third and seventh days post-treatment to assess malondialdehyde and superoxide dismutase expression. Statistical analyses included the Shapiro-Wilk test, Levene test, ANOVA, and Tukey’s HSD. Results: The samples treated with propolis extract-Ca(OH)2 combination exhibited significantly lower malondialdehyde expression on both days compared to samples treated with Ca(OH)2 (P<0.05), indicating reduced oxidative stress. Superoxide dismutase expression in the propolis extract-Ca(OH)2 group was higher (P<0.05), suggesting an enhanced antioxidant activity. The control group showed intermediate results. Statistical analyses confirmed significant differences between groups for both malondialdehyde and superoxide dismutase expressions (P<0.05). Conclusion: The study suggests that the propolis extract-Ca(OH)2 combination holds promise for direct pulp capping applications by minimizing oxidative stress and promoting antioxidant defense mechanisms in dental pulp.
AB - Introduction: This study explored the effects of propolis extract calcium hydroxide (Ca(OH)2 ) combination on malondialdehyde and superoxide dismutase expression in dental pulp, aiming to assess its potential as a direct pulp capping material. Materials and Methods: Thirty male Wistar rats were randomly assigned to three groups. Mandibular molar teeth were prepared using a low-speed round bur. In Group I, no material was applied; in Group II, teeth were treated with Ca(OH)2; and in Group III, teeth were treated with Propolis extract-Ca(OH)2, followed by Cention N filling. Immunohistochemistry was conducted on pulp tissue samples obtained on the third and seventh days post-treatment to assess malondialdehyde and superoxide dismutase expression. Statistical analyses included the Shapiro-Wilk test, Levene test, ANOVA, and Tukey’s HSD. Results: The samples treated with propolis extract-Ca(OH)2 combination exhibited significantly lower malondialdehyde expression on both days compared to samples treated with Ca(OH)2 (P<0.05), indicating reduced oxidative stress. Superoxide dismutase expression in the propolis extract-Ca(OH)2 group was higher (P<0.05), suggesting an enhanced antioxidant activity. The control group showed intermediate results. Statistical analyses confirmed significant differences between groups for both malondialdehyde and superoxide dismutase expressions (P<0.05). Conclusion: The study suggests that the propolis extract-Ca(OH)2 combination holds promise for direct pulp capping applications by minimizing oxidative stress and promoting antioxidant defense mechanisms in dental pulp.
KW - Calcium Hydroxide
KW - Direct Pulp Capping
KW - Malondialdehyde
KW - Propolis
KW - Superoxide Dismutase
UR - http://www.scopus.com/inward/record.url?scp=85188682781&partnerID=8YFLogxK
U2 - 10.22037/iej.v19i2.42269
DO - 10.22037/iej.v19i2.42269
M3 - Article
AN - SCOPUS:85188682781
SN - 1735-7497
VL - 19
SP - 99
EP - 104
JO - Iranian Endodontic Journal
JF - Iranian Endodontic Journal
IS - 2
ER -