Immunomodulatory effect of propolis extract on granzyme expression in cd8+ and cd4+cd25+ t cells

Zauhani Kusnul, Pudji Rahayu, Muhaimin Rifai, Edi Widjajanto

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Objective: The purpose of this study was to explore the effect of propolis extract on CD8+ and CD4+CD25+ regulatory T cell populations and granzyme expression in both cell populations, as propolis has been suggested as an immunomodulatory agent. Materials and Methods: PBMC containing 1x106 cells/ml from cervical cancer stage 3 patients were isolated and cultured with and without propolis extract. The cells were plated in a 24-well plate with RPMI medium supplemented with 10% FBS, 10 U/ml penicillin, 100 U/ml streptomycin, 2 mM L-glutamine and 50 μM 2-ME. Propolis extract was added (5, 10, 25 and 50 μg/ml) to PBMC culture. Negative control was made from PBMC culture without propolis extract for comparison. The cells were incubated for 48 hours in a 5% CO2 incubator at 37ºC. The proportion of CD8+ and CD4+CD25+ regulatory T cells that expressed granzyme were measured by flow cytometry. Results: The results obtained that the level of CD4+, CD4+CD25+, and CD8+ T cells were not markedly depleted in propolis treatment in all doses compared to control group. The percentage of CD8+Granzyme+ and CD4+CD25+Granzyme+ were significantly different in groups of propolis treatment compared to control group. Conclusion: In summary, propolis extract may serve as an immunomodulatory agent in cervical cancer patients. Propolis extract could modulate the expression CD4+CD25+ T cells expressing granzyme and CD8+ T cells expressing granzyme while it has no effect on CD4+, CD4+CD25+, and CD8+ T cells.

Original languageEnglish
Pages (from-to)13-19
Number of pages7
JournalTurkish Journal of Immunology
Issue number1
Publication statusPublished - 2017
Externally publishedYes


  • CD4CD25 regulatory T cells
  • CD8 T cells
  • Granzyme
  • Propolis


Dive into the research topics of 'Immunomodulatory effect of propolis extract on granzyme expression in cd8+ and cd4+cd25+ t cells'. Together they form a unique fingerprint.

Cite this