Proliferator activity, expression interleukin-2 andinterferon- γ of immunoglobulin Y anti HIV in peripheral blood mononuclear cells

Sri Agus Sudjarwo, Giftania Wardani Sudjarwo, O. Koerniasari

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The present study was performed to investigate the effect of Immunoglobulin (Ig)Y anti HIVon PHA-activated peripheral blood mononuclear cells (PBMCs) proliferation, interleukin (IL)-2 and interferon (IFN)-γ secretion. Effects of IgY anti HIV in different concentrations of 25, 50 and 100 μg/mlon phytohemagglutinin (PHA) activated-human peripheral blood mononuclearcells (PBMC) proliferation were determined byMTT assay. The concentration of IL-2 and IFN-γ in the PBMCs supernatant was determined using enzymelinked immunosorbent assay (ELISA). Determination of mRNA expression of IL-2 and IFN-γ by reverse transcription-polymerase chain reaction (RT-PCR).IgY anti HIVsignificantly enhanced the proliferation of PBMC induced by PHA. Furthermore, IgY anti HIV dosedependently enhanced IL-2 and IFN-γ production in PHA-activated PBMC, suggestingthat pharmacological activities of Ig Y anti HIV inPHA-activated PBMC may be mediated by regulating the production ofcytokines in PBMC. In the RT-PCR, production of cytokines such as IL-2 and IFN-γ in PHA-activated PBMC cultures were increased by IgY anti HIV. We concluded that the enhancements of IL-2 and IFN-γproductions in PHA-activated PBMC were related to IgY anti HIV stimulating the mRNA transcription of these cytokines.IgY anti HIVincreased IL-2 and IFN-γ productions in PHA-activated PBMC cultures by modulation of their gene expression. Cell proliferation, IL-2 and IFN-γ production of PBMC play important roles against bacterial and viral infection. It suggests that IgY anti HIV may be an immunotherapy. However, its detailed mechanisms of action are subjected for further study.

Original languageEnglish
Article numberA024
Pages (from-to)194-199
Number of pages6
JournalInternational Journal of ChemTech Research
Volume8
Issue number10
Publication statusPublished - 2015

Keywords

  • Human PBMCs
  • IFN-
  • IL-2
  • IgY anti HIV

Fingerprint

Dive into the research topics of 'Proliferator activity, expression interleukin-2 andinterferon- γ of immunoglobulin Y anti HIV in peripheral blood mononuclear cells'. Together they form a unique fingerprint.

Cite this