Proliferator activity, expression interleukin-2 andinterferon- γ of immunoglobulin Y anti HIV in peripheral blood mononuclear cells

The present study was performed to investigate the effect of Immunoglobulin (Ig)Y anti HIVon PHA-activated peripheral blood mononuclear cells (PBMCs) proliferation, interleukin (IL)-2 and interferon (IFN)-_γ secretion. Effects of IgY anti HIV in different concentrations of 25, 50 and 100 μg/mlon phytohemagglutinin (PHA) activated-human peripheral blood mononuclearcells (PBMC) proliferation were determined byMTT assay. The concentration of IL-2 and IFN-γ in the PBMCs supernatant was determined using enzymelinked immunosorbent assay (ELISA). Determination of mRNA expression of IL-2 and IFN-_γ by reverse transcription-polymerase chain reaction (RT-PCR).IgY anti HIVsignificantly enhanced the proliferation of PBMC induced by PHA. Furthermore, IgY anti HIV dosedependently enhanced IL-2 and IFN-_γ production in PHA-activated PBMC, suggestingthat pharmacological activities of Ig Y anti HIV inPHA-activated PBMC may be mediated by regulating the production ofcytokines in PBMC. In the RT-PCR, production of cytokines such as IL-2 and IFN-_γ in PHA-activated PBMC cultures were increased by IgY anti HIV. We concluded that the enhancements of IL-2 and IFN-_γproductions in PHA-activated PBMC were related to IgY anti HIV stimulating the mRNA transcription of these cytokines.IgY anti HIVincreased IL-2 and IFN-γ productions in PHA-activated PBMC cultures by modulation of their gene expression. Cell proliferation, IL-2 and IFN-_γ production of PBMC play important roles against bacterial and viral infection. It suggests that IgY anti HIV may be an immunotherapy. However, its detailed mechanisms of action are subjected for further study.