Abstract
The role of micro Ribo Nucleic Acids (miRNA), a small-non coding RNA has been associated with immune regulation in various viral infection including dengue infection. The microRNA will bind a specific protein target in order to encourage an explosive expression of various cytokines, known as cytokines storm in Dengue infection. The objective of this study aimed to determine and evaluate the microRNAs profile expression within peripheral blood mononuclear cells having been infected with one of the dengue virus serotype. To obtained the PBMCs from a healthy donor, Ficoll density gradient centrifugation was used to isolate the PBMCs and then followed infecting it with a DENV-2 clinical isolate. Prior to PBMCs isolation, the virus has been propagated and having titration to get an optimal virus titer. We conducted the infection at the multiplication of infections 4 PFU/106 cells. MiRCURY LNATMExiqon was utilized on purpose to extract the RNA. Quantitative Real-Time PCR was applied in order for the miRNAs relative expression to be measured. The preliminary result reveals that miR-150, miR-146a, hsa-let-7e expression were increased 1.74 folds, 2 folds, and 1.49 folds respectively at 12 hours post-infection on PBMCs upon DENV-2 infection. The expression of microRNAs was discovered to be higher in PBMCs at the time of infection with DENV-2. The miRNAs expression in the uninfected PMBCs was lower than that of the miRNA. This high expression of miRNAs in dengue infection may proceed to dengue infection pathogenesis.
Original language | English |
---|---|
Pages (from-to) | 923-927 |
Number of pages | 5 |
Journal | Biomedical and Pharmacology Journal |
Volume | 11 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2017 |
Keywords
- Dengue
- Expression
- Infection.
- MiRNA