Escherichia coli is one of the pathogenic bacteria that caused a nosocomial infection. Levofloxacin is one of the fluoroquinolones group antibiotics which is a broad-spectrum antibiotic that works effectively against E. coli. The mutation can happen in the bacteria which caused a resistant effect in the use of antibacterial. This study aimed at identifying mutation in gene gyrA among E. coli that were resistant to levofloxacin. The susceptibility of E. coli was determined by disk diffusion. PCR and sequencing were performed to identify the mutation in gyrA. A total of 10 isolates showed result resistance to levofloxacin and gyrA gene mutation in the amino acid changes. Nucleotide sequence analysis revealed a point mutation in QRDR (quinolone resistance determining region) of gyrA Ser83→Leu, Asp87→Asn. The silent mutation was also found at codon Val85, Arg91, Ser111, Thr123. Mutation in the gyrA gene affect the occurrence of bacterial resistance of E. coli to levofloxacin.
|Number of pages||4|
|Journal||Journal of Basic and Clinical Physiology and Pharmacology|
|Publication status||Published - 1 Jul 2021|
- Escherichia coli