Production of recombinant dengue non-structural 1 (NS1) proteins from clinical virus isolates

Benediktus Yohan; Puspa Wardhani; Hidayat Trimarsanto; R. Tedjo Sasmono

doi:10.1016/j.pep.2016.09.007

Production of recombinant dengue non-structural 1 (NS1) proteins from clinical virus isolates

Benediktus Yohan, Puspa Wardhani, Aryati, Hidayat Trimarsanto, R. Tedjo Sasmono

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Dengue is a febrile disease caused by infection of dengue virus (DENV). Early diagnosis of dengue infection is important for better management of the disease. The DENV Non-Structural Protein 1 (NS1) antigen has been routinely used for the early dengue detection. In dengue epidemic countries such as Indonesia, clinicians are increasingly relying on the NS1 detection for confirmation of dengue infection. Various NS1 diagnostic tests are commercially available, however different sensitivities and specificities were observed in various settings. This study was aimed to generate dengue NS1 recombinant protein for the development of dengue diagnostic tests. Four Indonesian DENV isolates were used as the source of the NS1 gene cloning, expression, and purification in bacterial expression system. Recombinant NS1 proteins were successfully purified and their antigenicities were assessed. Immunization of mice with recombinant proteins observed the immunogenicity of the NS1 protein. The generated recombinant proteins can be potentially used in the development of NS1 diagnostic test. With minimal modifications, this method can be used for producing NS1 recombinant proteins from isolates obtained from other geographical regions.

Original language	English
Pages (from-to)	53-59
Number of pages	7
Journal	Protein Expression and Purification
Volume	129
DOIs	https://doi.org/10.1016/j.pep.2016.09.007
Publication status	Published - 1 Jan 2017

Keywords

Dengue
Diagnostic
NS1
Recombinant protein

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.pep.2016.09.007

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title = "Production of recombinant dengue non-structural 1 (NS1) proteins from clinical virus isolates",

abstract = "Dengue is a febrile disease caused by infection of dengue virus (DENV). Early diagnosis of dengue infection is important for better management of the disease. The DENV Non-Structural Protein 1 (NS1) antigen has been routinely used for the early dengue detection. In dengue epidemic countries such as Indonesia, clinicians are increasingly relying on the NS1 detection for confirmation of dengue infection. Various NS1 diagnostic tests are commercially available, however different sensitivities and specificities were observed in various settings. This study was aimed to generate dengue NS1 recombinant protein for the development of dengue diagnostic tests. Four Indonesian DENV isolates were used as the source of the NS1 gene cloning, expression, and purification in bacterial expression system. Recombinant NS1 proteins were successfully purified and their antigenicities were assessed. Immunization of mice with recombinant proteins observed the immunogenicity of the NS1 protein. The generated recombinant proteins can be potentially used in the development of NS1 diagnostic test. With minimal modifications, this method can be used for producing NS1 recombinant proteins from isolates obtained from other geographical regions.",

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author = "Benediktus Yohan and Puspa Wardhani and Aryati and Hidayat Trimarsanto and Sasmono, {R. Tedjo}",

note = "Funding Information: This study was funded by SINAS Ristek grant 2012–2014 from the Ministry of Research, Technology, and Higher Education of the Republic of Indonesia . The funder has no involvement in study design: in the collection, analysis and interpretation of data; in the writing of the report; and in the decision to submit the article for publication. We are grateful for the technical helps of T. Y. Setianingsih. The help from PT Bimana Indomedical in mouse experimentation is greatly appreciated. Publisher Copyright: {\textcopyright} 2016 Elsevier Inc.",

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AU - Sasmono, R. Tedjo

N1 - Funding Information: This study was funded by SINAS Ristek grant 2012–2014 from the Ministry of Research, Technology, and Higher Education of the Republic of Indonesia . The funder has no involvement in study design: in the collection, analysis and interpretation of data; in the writing of the report; and in the decision to submit the article for publication. We are grateful for the technical helps of T. Y. Setianingsih. The help from PT Bimana Indomedical in mouse experimentation is greatly appreciated. Publisher Copyright: © 2016 Elsevier Inc.

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N2 - Dengue is a febrile disease caused by infection of dengue virus (DENV). Early diagnosis of dengue infection is important for better management of the disease. The DENV Non-Structural Protein 1 (NS1) antigen has been routinely used for the early dengue detection. In dengue epidemic countries such as Indonesia, clinicians are increasingly relying on the NS1 detection for confirmation of dengue infection. Various NS1 diagnostic tests are commercially available, however different sensitivities and specificities were observed in various settings. This study was aimed to generate dengue NS1 recombinant protein for the development of dengue diagnostic tests. Four Indonesian DENV isolates were used as the source of the NS1 gene cloning, expression, and purification in bacterial expression system. Recombinant NS1 proteins were successfully purified and their antigenicities were assessed. Immunization of mice with recombinant proteins observed the immunogenicity of the NS1 protein. The generated recombinant proteins can be potentially used in the development of NS1 diagnostic test. With minimal modifications, this method can be used for producing NS1 recombinant proteins from isolates obtained from other geographical regions.

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Production of recombinant dengue non-structural 1 (NS1) proteins from clinical virus isolates

Abstract

Keywords

UN SDGs

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