TY - JOUR
T1 - Production of biosurfactant by Arthrobacter sp. P2(1) in the carbohydrate-containing medium
AU - Fatimah,
AU - Suharjono,
AU - Ardyati, Tri
AU - Ni’Matuzahroh,
N1 - Publisher Copyright:
© 2016, Sphinx Knowledge House. All rights reserved.
PY - 2016
Y1 - 2016
N2 - Surfactants are surface active molecules which have ability to reduce the surface and interfacial tension between the two liquid phases. Biosurfactants, which is produced by living cells. Have more effective, selective, stable, and environmental-friendly than chemical synthesized surfactants. However, the producing cost of biosurfactants are tends to more expensive. Furthermore, inexpensive substrate selection and indigenous biological highproductivity producer strains is one way to reduce the production cost. The purpose of this study was to determine the ability of one of indigeneous petroleum-contaminated soil bacteria, Arthrobacter sp.P2(1), in producing biosurfactant in three types of carbohydrate substrates, such as High Fructose Syrup (HFS), sucrose, and molasses. Therefore, Arthrobacter sp. P2(1) was grown in mineral synthetic medium containing 2% carbohydrate. Cultures were incubated for 4 days and their surfactant producing ability was observed. The surface tension decrease of culture supernatant was measured using a Du Nouy tensiometer. Hydrocarbon emulsifying activity of supernatant was assayed using diesel oil and kerosene. Arthrobacter sp. P2(1) can grow well and produce bisurfactant in the three carbohydrate substrates and growth optimally in molasses-containing medium. Arthrobacter sp. P2(1) produce biosurfactant which has property as an emulsifier in the Hfs and sucrose-containing medium. Furthermore, Arthrobacter sp.P2(1) produce biosurfactant which has properties as surface active agent and emulsifiers in the molasses-containing medium.
AB - Surfactants are surface active molecules which have ability to reduce the surface and interfacial tension between the two liquid phases. Biosurfactants, which is produced by living cells. Have more effective, selective, stable, and environmental-friendly than chemical synthesized surfactants. However, the producing cost of biosurfactants are tends to more expensive. Furthermore, inexpensive substrate selection and indigenous biological highproductivity producer strains is one way to reduce the production cost. The purpose of this study was to determine the ability of one of indigeneous petroleum-contaminated soil bacteria, Arthrobacter sp.P2(1), in producing biosurfactant in three types of carbohydrate substrates, such as High Fructose Syrup (HFS), sucrose, and molasses. Therefore, Arthrobacter sp. P2(1) was grown in mineral synthetic medium containing 2% carbohydrate. Cultures were incubated for 4 days and their surfactant producing ability was observed. The surface tension decrease of culture supernatant was measured using a Du Nouy tensiometer. Hydrocarbon emulsifying activity of supernatant was assayed using diesel oil and kerosene. Arthrobacter sp. P2(1) can grow well and produce bisurfactant in the three carbohydrate substrates and growth optimally in molasses-containing medium. Arthrobacter sp. P2(1) produce biosurfactant which has property as an emulsifier in the Hfs and sucrose-containing medium. Furthermore, Arthrobacter sp.P2(1) produce biosurfactant which has properties as surface active agent and emulsifiers in the molasses-containing medium.
KW - Biosurfactant
KW - Carbohydrate
KW - Emulsification
KW - Surface tension
UR - http://www.scopus.com/inward/record.url?scp=84968756779&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:84968756779
SN - 0974-4290
VL - 9
SP - 644
EP - 649
JO - International Journal of ChemTech Research
JF - International Journal of ChemTech Research
IS - 4
ER -