Procalcitonin, IL-1β, HSP10 and resolvin D2 mechanism as sepsis biomarkers in sepsis model

Background: The use of various biomarkers in sepsis has an important role in helping diagnosis and decision making therapy quickly and accurately. This study aimed to explain the mechanism of procalcitonin, IL-1β, RAMPs markers such as HSP10 and resolvin D2 as biomarkers of sepsis in the sepsis model of Eschericia coli infection given with meropenem antibiotics. Methods: An experimental study using the E. coli sepsis model in male Rattus norvegicuswas conducted in 2 stages. The first step was to observe a model of sepsis in rats and determine the time of sepsis and the second step was to compare levels of procalcitonin, HSP10, IL-1β, and resolvin D2 with different dosage of germs and different antibiotic time of administration. Results: The time of sepsis was occurred between 8 and 10 hours. There were differences in E. coli exposure with IL-1β levels (p = 0.03), procalcitonin (p = 0.084), HSP10 (p = 0.000), and resolvin D2 (p = 0.024). There were differences in levels of procalcitonin and resolvin D2 between groups at 24, 48 and 72 hours and between the treatment of antibiotics at different times and without antibiotic administration. While there were differences the levels of IL-1β and HSP10 between groups at 24 and 72 hours, but there was no difference at 48 hours antibiotic treatments. Conclusion: Resolvin D2 and HSP10 have a slightly different pattern at 24 and 72 hours compared to procalcitonin and HSP10. Thus, it has the potential to be developed as a new biomarker in sepsis.