PREVENTIVE EFFECTS OF Apis dorsata HONEY ON THE SPERMATOGENIC CELLS AND SERTOLI CELLS COUNT OF MICE (Mus Musculus) EXPOSED TO MONOSODIUM GLUTAMATE

The purpose of this study was to determine the preventive effect of Apis dorsata honey (AH) on the spermatogenic and Sertoli cells count of mice (Mus musculus) exposed to monosodium glutamate (MSG). This study used 25 male BALB/c mice which were divided into five treatments. The negative control (C-) received aquadest, the positive control (C+) received MSG 4 mg/gBW, T1 received AH 2.7 mg/gBW + MSG 4 mg/gBW, T2 received AH 5.4 mg/gBW + MSG 4 mg/gBW, and T3 received AH 8.1 mg/gBW and MSG 4 mg/gBW. All groups were treated for 52 days. The testes were then prepared as histopathology slides and examined under a microscope. The results of this study showed that there was a significant difference in spermatogenic cells and Sertoli cells between C-, C+, T1, and T2 (p<0.05) and showed no significant difference (p>0.05) in spermatid and Sertoli cells count between C- (90.28 ± 1.361 and 13.60 ± 0.374) and T3 treatment group (88.04 ± 1.212 and 13.04 ± 0.434). The best preventive dosage of AH was the T3 with 8.1 mg/gBW. It can be concluded that administration of AH can maintain spermatogenic and Sertoli cells count in mice exposed to MSG.