TY - JOUR
T1 - Prevalence of the blaCTX-M and blaTEM genes among extended-spectrum beta lactamase–producing Escherichia coli isolated from broiler chickens in Indonesia
AU - Faridah, Hayyun Durrotul
AU - Wibisono, Freshindy Marissa
AU - Wibisono, Freshinta Jellia
AU - Nisa, Nabilatun
AU - Fatimah, Fatimah
AU - Effendi, Mustofa Helmi
AU - Ugbo, Emmanuel Nnabuike
AU - Khairullah, Aswin Rafif
AU - Kurniawan, Shendy Canadya
AU - Silaen, Otto Sahat Martua
N1 - Publisher Copyright:
© 2023 H.D. Faridah et al.
PY - 2023
Y1 - 2023
N2 - Introduction: Infections of humans and animals by multidrug resistant bacteria are increasing because of the inappropriate use of antibiotics. Disease management may be more challenging if Escherichia coli produce extended-spectrum beta-lactamase (ESBL), which could cause resistance to aztreonam and third-generation cephalosporins. This study was aimed at determining the prevalence of the blaCTX-Mand blaTEM genes among ESBL-producing E. coli isolated from broiler chickens in Indonesia. Material and Methods: A total of 115 broiler cloacal swab samples were obtained from 22 farms and studied for the presence of E. coli. The isolates were identified using approved standard methods and were purified on eosin methylene blue agar media. The E. coli isolates were subjected to sensitivity testing using beta-lactam antibiotics, and ESBL production was confirmed by a double-disc synergy test. The presence of the blaCTX-Mand blaTEM genes was identified using a PCR. Results: It was found that 99/115 (86.1%) of the isolated E. coli were resistant to beta-lactam antibiotics and 34/115 (29.6%) of them were phenotypically detected to be ESBL producers. Of the 34 isolates that were confirmed ESBL producers, 32/34 (94.1%) of them harboured the blaCTX-Mand 13/34 (38.2%) the blaTEM genes. The blaCTX-Mand blaTEM genes were detected together in 12/34 (35.3%) isolates. Conclusion: This study discovered that broiler chickens are possible reservoirs of ESBL-producing E. coli that may infect humans. Thus, a committed public health education campaign is recommended in order to mitigate the potential threat to human health.
AB - Introduction: Infections of humans and animals by multidrug resistant bacteria are increasing because of the inappropriate use of antibiotics. Disease management may be more challenging if Escherichia coli produce extended-spectrum beta-lactamase (ESBL), which could cause resistance to aztreonam and third-generation cephalosporins. This study was aimed at determining the prevalence of the blaCTX-Mand blaTEM genes among ESBL-producing E. coli isolated from broiler chickens in Indonesia. Material and Methods: A total of 115 broiler cloacal swab samples were obtained from 22 farms and studied for the presence of E. coli. The isolates were identified using approved standard methods and were purified on eosin methylene blue agar media. The E. coli isolates were subjected to sensitivity testing using beta-lactam antibiotics, and ESBL production was confirmed by a double-disc synergy test. The presence of the blaCTX-Mand blaTEM genes was identified using a PCR. Results: It was found that 99/115 (86.1%) of the isolated E. coli were resistant to beta-lactam antibiotics and 34/115 (29.6%) of them were phenotypically detected to be ESBL producers. Of the 34 isolates that were confirmed ESBL producers, 32/34 (94.1%) of them harboured the blaCTX-Mand 13/34 (38.2%) the blaTEM genes. The blaCTX-Mand blaTEM genes were detected together in 12/34 (35.3%) isolates. Conclusion: This study discovered that broiler chickens are possible reservoirs of ESBL-producing E. coli that may infect humans. Thus, a committed public health education campaign is recommended in order to mitigate the potential threat to human health.
KW - ESBL-producing E. coli
KW - bla
KW - bla
KW - broiler chickens
KW - human health
UR - http://www.scopus.com/inward/record.url?scp=85167426145&partnerID=8YFLogxK
U2 - 10.2478/jvetres-2023-0025
DO - 10.2478/jvetres-2023-0025
M3 - Article
AN - SCOPUS:85167426145
SN - 2450-7393
VL - 67
SP - 179
EP - 186
JO - Journal of Veterinary Research (Poland)
JF - Journal of Veterinary Research (Poland)
IS - 2
ER -