A study was conducted to isolate E. coli from raw milk samples and to determine the antibiogram pattern of E. coli isolates. A total of 150 milk samples were collected from East Java Province, Indonesia, and tested with CMT for detection of subclinical mastitis. MacConkey agar and Eosin Methylene agar were used as culture media. Several biochemical tests and Antibiogram pattern of E. coli by the disk diffusion method were used. Results of the present study have revealed that out of the 150 samples tested, 128 (85.33%) were found to have subclinical mastitis due to E. coli 94 (74.33%). Antibiogram pattern assessed, revealed a higher resistance against erythromycin (100%) and gentamicin (26%), followed by oxytetracycline (17.78%), and chloramphenicol (0%). The presence of antimicrobial resistance indicates alarming situation for timely designing of control and prevention methods to reduce its prevalence and antimicrobial resistance in foods of animal origin.

Original languageEnglish
Pages (from-to)22-25
Number of pages4
JournalIndian Veterinary Journal
Issue number3
Publication statusPublished - 2019


  • Antimicrobial resistance
  • E. coli
  • Raw milk
  • Subclinical mastitis


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