TY - JOUR
T1 - Prevalence of multidrug resistance and extended-spectrum beta-lactamase-producing Klebsiella pneumoniae from dairy cattle farm wastewater in East Java Province, Indonesia
AU - Dameanti, Fidi Nur Aini Eka Puji
AU - Yanestria, Sheila Marty
AU - Widodo, Agus
AU - Effendi, Mustofa Helmi
AU - Plumeriastuti, Hani
AU - Tyasningsih, Wiwiek
AU - Ugbo, Emmanuel Nnabuike
AU - Sutrisno, Rahayu
AU - Syah, M. Ali Akram
N1 - Publisher Copyright:
© 2023 Dameanti, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. All Rights Reserved.
PY - 2023
Y1 - 2023
N2 - Background and Aim: Antibiotic resistance in Klebsiella pneumoniae, especially extended-spectrum bcta-lactamasc (ESBL) producers, has become a global public health problem. This study aimed to estimate the prevalence of multidrug resistance (MDR) and ESBL-producing K. pneumoniae in wastewater from dairy farms. Materials and Methods: This study was conducted on dairy farms in East Java Province from June to October 2022. In total, 342 dairy farm wastewater samples were isolated on buffered peptone water media from six citics/rcgcncics with the highest dairy cattle population in East Java. Samples were identified using MacConkcy agar media, Gram-staining,cosin-mcthylcnc blue agar, and biochemical tests. In total, 14.32% (49/342) samples contained K. pneumoniae. Positive isolates were tested for antibiotic sensitivity. Klebsiella pneumoniae resistant to beta-lactam was confirmed using the double-disk synergy test to confirm the presence of ESBL-producing bacteria. Results: The percentage of antibiotic resistance in K. pneumoniae was 98% resistance to ampicillin, 67.3% to cefotaxime, 46.9% to tetracycline, 49% to ciprofloxacin, 98% to streptomycin, 14.3% to sulfamcthoxazolc-trimcthoprim, and 83.7% to chloramphenicol. The prevalence of MDR in K. pneumoniae was 12.57% (43/342), with the highest prevalence in the five classes of antibiotics at 41.86% (18/43), and the prevalence of ESBL-producing K. pneumoniae was 5.55% (19/342), with the highest prevalence in the districts of Blitar and Pasuruan at 26.31% (5/19). Conclusion: Although the prevalence of ESBL-producing K. pneumoniae in wastewater samples from dairy farms was low, caution is recommended because they can be a reservoir for ESBL.
AB - Background and Aim: Antibiotic resistance in Klebsiella pneumoniae, especially extended-spectrum bcta-lactamasc (ESBL) producers, has become a global public health problem. This study aimed to estimate the prevalence of multidrug resistance (MDR) and ESBL-producing K. pneumoniae in wastewater from dairy farms. Materials and Methods: This study was conducted on dairy farms in East Java Province from June to October 2022. In total, 342 dairy farm wastewater samples were isolated on buffered peptone water media from six citics/rcgcncics with the highest dairy cattle population in East Java. Samples were identified using MacConkcy agar media, Gram-staining,cosin-mcthylcnc blue agar, and biochemical tests. In total, 14.32% (49/342) samples contained K. pneumoniae. Positive isolates were tested for antibiotic sensitivity. Klebsiella pneumoniae resistant to beta-lactam was confirmed using the double-disk synergy test to confirm the presence of ESBL-producing bacteria. Results: The percentage of antibiotic resistance in K. pneumoniae was 98% resistance to ampicillin, 67.3% to cefotaxime, 46.9% to tetracycline, 49% to ciprofloxacin, 98% to streptomycin, 14.3% to sulfamcthoxazolc-trimcthoprim, and 83.7% to chloramphenicol. The prevalence of MDR in K. pneumoniae was 12.57% (43/342), with the highest prevalence in the five classes of antibiotics at 41.86% (18/43), and the prevalence of ESBL-producing K. pneumoniae was 5.55% (19/342), with the highest prevalence in the districts of Blitar and Pasuruan at 26.31% (5/19). Conclusion: Although the prevalence of ESBL-producing K. pneumoniae in wastewater samples from dairy farms was low, caution is recommended because they can be a reservoir for ESBL.
KW - Klebsiella pneumoniae
KW - animal health
KW - dairy cattle
KW - extended-spectrum bcta-lactamasc
KW - human health
KW - wastewater
UR - http://www.scopus.com/inward/record.url?scp=85183533478&partnerID=8YFLogxK
U2 - 10.14202/IJOH.2023.141-149
DO - 10.14202/IJOH.2023.141-149
M3 - Article
AN - SCOPUS:85183533478
SN - 2455-5673
VL - 9
SP - 141
EP - 149
JO - International Journal of One Health
JF - International Journal of One Health
IS - 2
ER -
