Background and Aim: Antibiotic resistance in Klebsiella pneumoniae, especially extended-spectrum bcta-lactamasc (ESBL) producers, has become a global public health problem. This study aimed to estimate the prevalence of multidrug resistance (MDR) and ESBL-producing K. pneumoniae in wastewater from dairy farms. Materials and Methods: This study was conducted on dairy farms in East Java Province from June to October 2022. In total, 342 dairy farm wastewater samples were isolated on buffered peptone water media from six citics/rcgcncics with the highest dairy cattle population in East Java. Samples were identified using MacConkcy agar media, Gram-staining,cosin-mcthylcnc blue agar, and biochemical tests. In total, 14.32% (49/342) samples contained K. pneumoniae. Positive isolates were tested for antibiotic sensitivity. Klebsiella pneumoniae resistant to beta-lactam was confirmed using the double-disk synergy test to confirm the presence of ESBL-producing bacteria. Results: The percentage of antibiotic resistance in K. pneumoniae was 98% resistance to ampicillin, 67.3% to cefotaxime, 46.9% to tetracycline, 49% to ciprofloxacin, 98% to streptomycin, 14.3% to sulfamcthoxazolc-trimcthoprim, and 83.7% to chloramphenicol. The prevalence of MDR in K. pneumoniae was 12.57% (43/342), with the highest prevalence in the five classes of antibiotics at 41.86% (18/43), and the prevalence of ESBL-producing K. pneumoniae was 5.55% (19/342), with the highest prevalence in the districts of Blitar and Pasuruan at 26.31% (5/19). Conclusion: Although the prevalence of ESBL-producing K. pneumoniae in wastewater samples from dairy farms was low, caution is recommended because they can be a reservoir for ESBL.

Original languageEnglish
Pages (from-to)141-149
Number of pages9
JournalInternational Journal of One Health
Issue number2
Publication statusPublished - 2023


  • Klebsiella pneumoniae
  • animal health
  • dairy cattle
  • extended-spectrum bcta-lactamasc
  • human health
  • wastewater


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