TY - JOUR
T1 - Prevalence and genotype distribution of hepatitis c virus among intravenous drug users (Idus) in Surabaya, Indonesia
AU - Humairah, Ira
AU - Lukitasari, Lina
AU - Handajani, Retno
AU - Amin, Mochamad
AU - Wungu, Citrawati Dyah Kencono
AU - Rianto, Edhi
AU - Ansori, Arif Nur Muhammad
N1 - Funding Information:
This study funded by Universitas Airlangga and the Ministry of Research, Technology, and Higher Education of the Republic of Indonesia. We thank the heads and staffs at Jagir Health Center, Surabaya, Methadone Clinic, Dr. Soetomo General Hospital, Surabaya, and the Institute of Tropical Disease (ITD),
Funding Information:
This study funded by Universitas Airlangga and the Ministry of Research, Technology, and Higher Education of the Republic of Indonesia. We thank the heads and staffs at Jagir Health Center, Surabaya, Methadone Clinic, Dr. Soetomo General Hospital, Surabaya, and the Institute of Tropical Disease (ITD), Universitas Airlangga, Surabaya.
Publisher Copyright:
© RJPT All right reserved.
PY - 2021/4
Y1 - 2021/4
N2 - Every year, there is an increase in the number Intravenous Drug Users (IDUs) in Indonesia, in which they have a greater risk of getting Hepatitis C Virus (HCV) infection. This study was aimed to determine HCV prevalence and genotypes among IDUs in Surabaya. A total 25 IDUs were tested for anti-HCV. Reverse-transcription PCR in the NS5B region and 5'UTR was carried out on serum with positive anti-HCV, continued with sequencing and HCV genotype/subtype determination. The prevalence of HCV infection in IDUs in Surabaya based on positive anti-HCV was 96%(24/25). Sequencing analysis showed that 92%(22/24) could be determined their genotypes and subtypes. 40.9% (9/22) samples had genotype 1 with subtype 1a 31.82%(7/22) and subtype 1c 9.09%(2/22). 40.9%(9/22) samples had genotype 3, with subtype 3a 18.18%(4/22) and 3k 22.73%(5/22). 13.64%(4/22) samples had genotype 4 with subtype 4a, and 4.55% (1/22) sample had genotype 6 subtype 6n. The prevalence of HCV infection was high among IDUs in Surabaya. In this study, genotypes 6 was found in which it is rarely found in Indonesia, so there is a possibility that there are different HCV genotype patterns in each region of Indonesia.
AB - Every year, there is an increase in the number Intravenous Drug Users (IDUs) in Indonesia, in which they have a greater risk of getting Hepatitis C Virus (HCV) infection. This study was aimed to determine HCV prevalence and genotypes among IDUs in Surabaya. A total 25 IDUs were tested for anti-HCV. Reverse-transcription PCR in the NS5B region and 5'UTR was carried out on serum with positive anti-HCV, continued with sequencing and HCV genotype/subtype determination. The prevalence of HCV infection in IDUs in Surabaya based on positive anti-HCV was 96%(24/25). Sequencing analysis showed that 92%(22/24) could be determined their genotypes and subtypes. 40.9% (9/22) samples had genotype 1 with subtype 1a 31.82%(7/22) and subtype 1c 9.09%(2/22). 40.9%(9/22) samples had genotype 3, with subtype 3a 18.18%(4/22) and 3k 22.73%(5/22). 13.64%(4/22) samples had genotype 4 with subtype 4a, and 4.55% (1/22) sample had genotype 6 subtype 6n. The prevalence of HCV infection was high among IDUs in Surabaya. In this study, genotypes 6 was found in which it is rarely found in Indonesia, so there is a possibility that there are different HCV genotype patterns in each region of Indonesia.
KW - Genotype
KW - Hepatitis C Virus
KW - Intravenous Drug Users
UR - http://www.scopus.com/inward/record.url?scp=85108257794&partnerID=8YFLogxK
U2 - 10.52711/0974-360X.2021.00354
DO - 10.52711/0974-360X.2021.00354
M3 - Article
AN - SCOPUS:85108257794
SN - 0974-3618
VL - 14
SP - 1999
EP - 2003
JO - Research Journal of Pharmacy and Technology
JF - Research Journal of Pharmacy and Technology
IS - 4
ER -