TY - JOUR
T1 - Preservation of wild isolates of human malaria parasites in wet ice and adaptation efficacy to in vitro culture
AU - Tantular, Indah S.
AU - Pusarawati, Suhintam
AU - Khin, Lin
AU - Kanbe, Toshio
AU - Kimura, Masatsugu
AU - Kido, Yasutoshi
AU - Kawamoto, Fumihiko
PY - 2012
Y1 - 2012
N2 - Wild isolates of malaria parasites were preserved in wet ice for 2-12 days and cultivated by a candle jar method. In four isolates of Plasmodium falciparum collected from Myanmar and preserved for 12 days, all failed to grow. In 31 isolates preserved for 5-10 days, nine were transformed to young gametocytes, but 22 isolates grew well. From Ranong, Thailand, nine isolates preserved for 7 days were examined, and six grew well. On the other hand, all of the 59 isolates collected from eastern Indonesian islands failed to establish as culture-adapted isolates, even most of them were preserved only for 2-3 days: 10 isolates stopped to grow, and 49 isolates were transformed to sexual stages by Day 10. These results indicated that a great difference in adaptation to in vitro culture may exist between wild isolates distributed in continental Southeast Asia and in eastern Indonesia and that gametocytogenesis might be easily switched on in Indonesian isolates. In wild isolates of P. vivax, P. malariae and P. ovale preserved for 2-9 days, ring forms or young trophozoites survived, but adaptation to in vitro culture failed. These results indicate that wild isolates can be preserved in wet ice for 9-10 days.
AB - Wild isolates of malaria parasites were preserved in wet ice for 2-12 days and cultivated by a candle jar method. In four isolates of Plasmodium falciparum collected from Myanmar and preserved for 12 days, all failed to grow. In 31 isolates preserved for 5-10 days, nine were transformed to young gametocytes, but 22 isolates grew well. From Ranong, Thailand, nine isolates preserved for 7 days were examined, and six grew well. On the other hand, all of the 59 isolates collected from eastern Indonesian islands failed to establish as culture-adapted isolates, even most of them were preserved only for 2-3 days: 10 isolates stopped to grow, and 49 isolates were transformed to sexual stages by Day 10. These results indicated that a great difference in adaptation to in vitro culture may exist between wild isolates distributed in continental Southeast Asia and in eastern Indonesia and that gametocytogenesis might be easily switched on in Indonesian isolates. In wild isolates of P. vivax, P. malariae and P. ovale preserved for 2-9 days, ring forms or young trophozoites survived, but adaptation to in vitro culture failed. These results indicate that wild isolates can be preserved in wet ice for 9-10 days.
KW - Gametocytogenesis
KW - Human malaria parasite
KW - In vitro culture
KW - Plasmodium
KW - Preservation
KW - Wet ice
KW - Wild isolate
UR - http://www.scopus.com/inward/record.url?scp=84867879268&partnerID=8YFLogxK
U2 - 10.2149/tmh.2012-07o
DO - 10.2149/tmh.2012-07o
M3 - Article
AN - SCOPUS:84867879268
SN - 1348-8945
VL - 40
SP - 37
EP - 45
JO - Tropical Medicine and Health
JF - Tropical Medicine and Health
IS - 2
ER -