TY - JOUR
T1 - Presence of multidrug resistance (MDR) and extended-spectrum beta-lactamase (ESBL) of Escherichia coli isolated from cloacal swabs of broilers in several wet markets in Surabaya, Indonesia
AU - Effendi, Mustofa Helmi
AU - Tyasningsih, Wiwiek
AU - Yurianti, Yemima Anggun
AU - Rahmahani, Jola
AU - Harijani, Nenny
AU - Plumeriastuti, Hani
N1 - Funding Information:
This study was supported in part with the Penelitian Hibah Mandat Funding from Airlangga University, Indonesia in fiscal year 2019.
Publisher Copyright:
© 2021, Society for Indonesian Biodiversity. All rights reserved.
PY - 2021/1
Y1 - 2021/1
N2 - Effendi MH, Tyasningsih W, Yurianti YA, Rahmahani J, Harijani N, Plumeriastuti H. 2020. Presence of multidrug resistance (MDR) and extended-spectrum beta-lactamase (ESBL) of Escherichia coli isolated from cloacal swab of broilers in several wet markets in Surabaya, Indonesia. Biodiversitas 22: 304-310. The purpose of this research was to identify multidrug resistance (MDR) and extended-spectrum beta-lactamase (ESBL) of Escherichia coli from cloacal swabs of broiler chicken in several wet markets in Surabaya. This study used 60 broiler chicken samples, with cloacal swab method. The samples were isolated and identified to find Escherichia coli with several procedures, including MacConckey Agar (MCA), Eosin Methylene Blue Agar (EMBA), Gram staining, indole test, Methyl Red-Voges Proskauer (MR-VP), citrate, and Triple Sugar Iron Agar (TSIA). Antibiotic sensitivity test was conducted by using Kirby-Bauer (Disc Diffusion Method) with antibiotics: Aztreonam, Gentamicin, Chloramphenicol, Tetracycline, and Ciprofloxacin. From results, it can be illustrated that the isolates resistant to antibiotic Ciprofloxacin were 67% and Tetracycline was 65%. Total 97% isolates were found sensitive for Aztreonam, 73% for Chloramphenicol, and 55% for Gentamicin. Twelve isolates identified for MDR and two were ESBL. It can be explained that broiler chicken from wet market should be considered as a source of transmission for MDR and ESBL of E. coli to the public health.
AB - Effendi MH, Tyasningsih W, Yurianti YA, Rahmahani J, Harijani N, Plumeriastuti H. 2020. Presence of multidrug resistance (MDR) and extended-spectrum beta-lactamase (ESBL) of Escherichia coli isolated from cloacal swab of broilers in several wet markets in Surabaya, Indonesia. Biodiversitas 22: 304-310. The purpose of this research was to identify multidrug resistance (MDR) and extended-spectrum beta-lactamase (ESBL) of Escherichia coli from cloacal swabs of broiler chicken in several wet markets in Surabaya. This study used 60 broiler chicken samples, with cloacal swab method. The samples were isolated and identified to find Escherichia coli with several procedures, including MacConckey Agar (MCA), Eosin Methylene Blue Agar (EMBA), Gram staining, indole test, Methyl Red-Voges Proskauer (MR-VP), citrate, and Triple Sugar Iron Agar (TSIA). Antibiotic sensitivity test was conducted by using Kirby-Bauer (Disc Diffusion Method) with antibiotics: Aztreonam, Gentamicin, Chloramphenicol, Tetracycline, and Ciprofloxacin. From results, it can be illustrated that the isolates resistant to antibiotic Ciprofloxacin were 67% and Tetracycline was 65%. Total 97% isolates were found sensitive for Aztreonam, 73% for Chloramphenicol, and 55% for Gentamicin. Twelve isolates identified for MDR and two were ESBL. It can be explained that broiler chicken from wet market should be considered as a source of transmission for MDR and ESBL of E. coli to the public health.
KW - Broiler chicken
KW - ESBL
KW - Escherichia coli
KW - MDR
KW - Public health
KW - Wet markets
UR - http://www.scopus.com/inward/record.url?scp=85098883532&partnerID=8YFLogxK
U2 - 10.13057/biodiv/d220137
DO - 10.13057/biodiv/d220137
M3 - Article
AN - SCOPUS:85098883532
SN - 1412-033X
VL - 22
SP - 304
EP - 310
JO - Biodiversitas
JF - Biodiversitas
IS - 1
ER -
