Preconditioning of hypoxic culture increases the therapeutic potential of adipose derived mesenchymal stem cells

Tito Sumarwoto, Heri Suroto, Ferdiansyah Mahyudin, Dwikora Novembri Utomo, Romaniyanto, Andhi Prijosedjati, Pamudji Utomo, Cita Rosita Sigit Prakoeswa, Fedik Abdul Rantam, Damayanti Tinduh, Hari Basuki Notobroto, Sholahuddin Rhatomy

Research output: Contribution to journalArticlepeer-review

Abstract

Stem cell-based therapy is one of the most promising strategies in promoting tissue regeneration. Mesenchymal stem cells (MSCs) derived from adipose are widely used as a source of stem cells. However, most donor stem cells die before their regenerative effects appear. Thereby, various strategies are carried out to enhance the therapeutic potential. It is hoped that the survival, engraftment, and function of implanted MSCs by preconditioning the cells will be improved. In vitro preconditioning strategies can improve the survival, engraftment, and paracrine properties of MSCs, thus optimize their reparative and regenerative capacities. One such attempt is to propagate these stem cells in a hypoxic environment, which mimics the natural tissue oxygen environment. Hypoxia preconditioning represents a comprehensive increase in regenerative gene expression in stem cell therapy. There are significant advantages in stem cell therapy with hypoxia preconditioning.

Original languageEnglish
Pages (from-to)505-515
Number of pages11
JournalOpen Access Macedonian Journal of Medical Sciences
Volume9
DOIs
Publication statusPublished - 10 Jan 2021

Keywords

  • Adipose Derived Mesenchymal Stem Cells
  • Culture
  • Hypoxic

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