TY - JOUR
T1 - Potential of Secretome - Hydrogel Based to Accelerate Bone Regeneration in Periodontitis
T2 - A Bioinformatics Approach
AU - Prasetyaningrum, Nenny
AU - Ridwan, Rini Devijanti
AU - Saskianti, Tania
AU - Rachmawati, Dini
AU - Aljunaid, Mohammed Ahmed
N1 - Publisher Copyright:
© (2024), (University of Dicle). All Rights Reserved.
PY - 2024
Y1 - 2024
N2 - Periodontitis can cause alveolar bone resorption. The secretome of stem cells from exfoliated primary teeth (SHED) contains TGF-β, which can stimulate osteogenesis in bone regeneration. Chitosan (CS)/β-glycerophosphate (BGP)/Hydroxyapatite (HA) based hydrogels have good biocompatibility. The research aims to predict the potential for adding a secretome to a hydrogel base by analysing protein-protein interactions (PPI) and binding interactions with several protein factors related to bone regeneration. The interaction and annotation of secretome with bone regeneration-related periodontitis were explored using BioGRID 4.4 data. The interaction analysis of CS/BGP/HA hydrogels was performed using DAVID, while for the PPI analysis, both SHED secretome and CS/BGP/HA hydrogels were analysed using STRING V.11.55 (https://string-db.org/). The molecular docking was performed using Autodock Vina integrated with Pyrx v.09.8. The results of the PPI analysis showed that BMP-2 was included in the top MCODE clustering. It was observed that TGF-β could activate BMP-2 with a prediction score of 0.8, indicating high confidence. Molecular docking results show that CS/BGP/HA ligands can interact individually with BMP-2, but their binding energies are lower than the threshold of -7 kcal/mol. Adding secretome to hydrogel can potentially increase bone regeneration through its binding interaction with BMP-2.
AB - Periodontitis can cause alveolar bone resorption. The secretome of stem cells from exfoliated primary teeth (SHED) contains TGF-β, which can stimulate osteogenesis in bone regeneration. Chitosan (CS)/β-glycerophosphate (BGP)/Hydroxyapatite (HA) based hydrogels have good biocompatibility. The research aims to predict the potential for adding a secretome to a hydrogel base by analysing protein-protein interactions (PPI) and binding interactions with several protein factors related to bone regeneration. The interaction and annotation of secretome with bone regeneration-related periodontitis were explored using BioGRID 4.4 data. The interaction analysis of CS/BGP/HA hydrogels was performed using DAVID, while for the PPI analysis, both SHED secretome and CS/BGP/HA hydrogels were analysed using STRING V.11.55 (https://string-db.org/). The molecular docking was performed using Autodock Vina integrated with Pyrx v.09.8. The results of the PPI analysis showed that BMP-2 was included in the top MCODE clustering. It was observed that TGF-β could activate BMP-2 with a prediction score of 0.8, indicating high confidence. Molecular docking results show that CS/BGP/HA ligands can interact individually with BMP-2, but their binding energies are lower than the threshold of -7 kcal/mol. Adding secretome to hydrogel can potentially increase bone regeneration through its binding interaction with BMP-2.
KW - Bone regeneration
KW - Hydrogel
KW - Molecular docking
KW - Secretome
UR - http://www.scopus.com/inward/record.url?scp=85190155148&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85190155148
SN - 1309-100X
VL - 17
SP - 136
EP - 144
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
IS - 1
ER -