TY - JOUR
T1 - Potential of antimicrobial-producing endophytic bacteria from Balikpapan endemic ginger (Etlingera balikpapanensis A.D. Poulsen)
AU - Fatimah,
AU - Asritafriha, Lillah
AU - Salsabila, Syarifah
AU - Dewi, Erna Kristiana
AU - Ni’matuzahroh,
AU - Geraldi, Almando
AU - Ramadhan, Rico
AU - Suwito, Hery
AU - Rahman, Arrizal
AU - Riyadi, Lukman
N1 - Publisher Copyright:
© 2024, Society for Indonesian Biodiversity. All rights reserved.
PY - 2024/9
Y1 - 2024/9
N2 - Etlingera balikpapanensis A.D. Poulsen is a new species of the Zingiberaceae family, which was discovered as an endemic species in the Balikpapan, Kalimantan. This study aimed to isolate endophytic bacteria from endemic Balikpapan ginger (E. balikpapanensis) and evaluate its antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Candida albicans and to identify the most promising antimicrobial isolates. Plant samples include roots, stems, and leaves. The process of isolating bacteria from various plant parts, such as roots, stems, and leaves, involved repeated subculturing until pure isolates were obtained. Seven pure isolates were successfully obtained, coded as 6D, 5R, 2R, 3D1, 10D, 4R2, and 2D. Macroscopic, microscopic, and biochemical characterization of the seven isolates was carried out. The seven endophytic bacteria had varied macroscopic characteristics and were Gram-positive. The disc diffusion method was used to determine the ability of isolates to produce antimicrobial agents. The isolates showed antibacterial activity against E. coli and S. aureus. Isolates 3D1 and 5R showed the highest antibacterial activity against E. coli (8.20±0.47 mm and 8.57±0.08 mm), and 5R also showed the highest antibacterial activity against S. aureus (8.14±0.49 mm). These results demonstrate the potential of these isolates as effective antimicrobial agents. The 16S rRNA gene analysis revealed that isolate 3D1 was identified as Bacillus cereus strain PJA4.2 and isolate 5R as Micrococcus luteus strain SGAir0127 with similarity of 99.55% and 100%, respectively.
AB - Etlingera balikpapanensis A.D. Poulsen is a new species of the Zingiberaceae family, which was discovered as an endemic species in the Balikpapan, Kalimantan. This study aimed to isolate endophytic bacteria from endemic Balikpapan ginger (E. balikpapanensis) and evaluate its antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Candida albicans and to identify the most promising antimicrobial isolates. Plant samples include roots, stems, and leaves. The process of isolating bacteria from various plant parts, such as roots, stems, and leaves, involved repeated subculturing until pure isolates were obtained. Seven pure isolates were successfully obtained, coded as 6D, 5R, 2R, 3D1, 10D, 4R2, and 2D. Macroscopic, microscopic, and biochemical characterization of the seven isolates was carried out. The seven endophytic bacteria had varied macroscopic characteristics and were Gram-positive. The disc diffusion method was used to determine the ability of isolates to produce antimicrobial agents. The isolates showed antibacterial activity against E. coli and S. aureus. Isolates 3D1 and 5R showed the highest antibacterial activity against E. coli (8.20±0.47 mm and 8.57±0.08 mm), and 5R also showed the highest antibacterial activity against S. aureus (8.14±0.49 mm). These results demonstrate the potential of these isolates as effective antimicrobial agents. The 16S rRNA gene analysis revealed that isolate 3D1 was identified as Bacillus cereus strain PJA4.2 and isolate 5R as Micrococcus luteus strain SGAir0127 with similarity of 99.55% and 100%, respectively.
KW - 16S rRNA gene
KW - Antimicrobial agent
KW - endemic ginger
KW - endophytic bacteria
KW - Etlingera balikpapanensis
UR - http://www.scopus.com/inward/record.url?scp=85208581661&partnerID=8YFLogxK
U2 - 10.13057/biodiv/d250922
DO - 10.13057/biodiv/d250922
M3 - Article
AN - SCOPUS:85208581661
SN - 1412-033X
VL - 25
SP - 3005
EP - 3013
JO - Biodiversitas
JF - Biodiversitas
IS - 9
ER -