TY - JOUR
T1 - Porous hydroxyapatite scaffold produced using Musa paradisiaca template and its in vitro bioactivity
AU - Fadli, Ahmad
AU - Widiyanti, Prihartini
AU - Noviana, Deni
AU - Prabowo, Agung
AU - Mulyadi, Adi
AU - Deska,
N1 - Publisher Copyright:
© 2021, Australian Ceramic Society.
PY - 2022/2
Y1 - 2022/2
N2 - The effect of HA amount on the physical properties and bioactivity of porous HA prepared by replica method using Musa paradisiaca as template has been studied. The templates were prepared by cutting the banana fronds into cylindrical shape. Slurries were prepared by mixing 9, 10, and 11 g HA with 11% sago starch, 2.5% Darvan821A, and distilled water. Slurries then stirred at 150 rpm for 24 h. Templates were impregnated into slurry and then dried at 110°C for 2 h. The green bodies were burned at 600°C for 1 h followed by sintering at 1250°C for 1 h. Porous HA was tested in vitro using simulated body fluid solution by soaking for 7–14 days. The cell attachment done by using Baby Hamster Kidney (BHK21) cell. The porous HA was produced with shrinkage in the range of 53.6–58.9%vol, density of 1.26–1.47 g/cm3, porosity of 53.5–60.1%, and compressive strength of 3.89–4 MPa. The pore size was obtained at the range 71.26–89.13 μm. The biodegradation rate of samples was found at the range of 1.34–2.27% with the increased apatite and carbonate content on porous HA after immersion proven by FTIR result. The cell attachment test showed that the viability of HA was 80.3% confirming that samples were non-toxic material.
AB - The effect of HA amount on the physical properties and bioactivity of porous HA prepared by replica method using Musa paradisiaca as template has been studied. The templates were prepared by cutting the banana fronds into cylindrical shape. Slurries were prepared by mixing 9, 10, and 11 g HA with 11% sago starch, 2.5% Darvan821A, and distilled water. Slurries then stirred at 150 rpm for 24 h. Templates were impregnated into slurry and then dried at 110°C for 2 h. The green bodies were burned at 600°C for 1 h followed by sintering at 1250°C for 1 h. Porous HA was tested in vitro using simulated body fluid solution by soaking for 7–14 days. The cell attachment done by using Baby Hamster Kidney (BHK21) cell. The porous HA was produced with shrinkage in the range of 53.6–58.9%vol, density of 1.26–1.47 g/cm3, porosity of 53.5–60.1%, and compressive strength of 3.89–4 MPa. The pore size was obtained at the range 71.26–89.13 μm. The biodegradation rate of samples was found at the range of 1.34–2.27% with the increased apatite and carbonate content on porous HA after immersion proven by FTIR result. The cell attachment test showed that the viability of HA was 80.3% confirming that samples were non-toxic material.
KW - Bioactivity
KW - Bioceramics
KW - Replica method
KW - Simulated body fluid
UR - http://www.scopus.com/inward/record.url?scp=85122090315&partnerID=8YFLogxK
U2 - 10.1007/s41779-021-00677-z
DO - 10.1007/s41779-021-00677-z
M3 - Article
AN - SCOPUS:85122090315
SN - 2510-1560
VL - 58
SP - 357
EP - 366
JO - Journal of the Australian Ceramic Society
JF - Journal of the Australian Ceramic Society
IS - 1
ER -