TY - JOUR
T1 - Phytochemicals of gandarusa (Justicia gendarussa) and its preparations
AU - Ratih, Gusti Ayu Made
AU - Imawati, Maria Fatmadewi
AU - Nugroho, Rendra Rizki
AU - Purwanti, Diah Intan
AU - Wongso, Suwidji
AU - Prajogo, Bambang
AU - Indrayanto, Gunawan
N1 - Publisher Copyright:
© The Author(s) 2019.
PY - 2019/6
Y1 - 2019/6
N2 - Thirty-five metabolites of Justicia gendarussa (JG) leaves and its preparations were identified using LC-HR-MS/MS. Although alkaloids were detected in the leaves they were not identified in JG preparations using Smart Formula 3D software. This showed that an acidified extraction process used at the first stage of the purification procedure is able to remove the toxic alkaloids from the crude drug. The LC-MS/MS analyses showed that the main components of JG preparations were fatty acids and apigenin glycosides; it seemed that the fatty acids can be used for enhancing the dissolution of the polar glycosides. T-test calculation using Profile Analysis software showed that the acidified crude drugs, extract, granules of JG, and gendarussa capsules showed very similar LC-MS/MS profiles, which means that the biochemical components of JG are relatively stable during processing. Due to the lack of quality markers for these JG preparations, the application of metabolite profiling is recommended as the QC tool for commercial production by the pharmaceutical industry.
AB - Thirty-five metabolites of Justicia gendarussa (JG) leaves and its preparations were identified using LC-HR-MS/MS. Although alkaloids were detected in the leaves they were not identified in JG preparations using Smart Formula 3D software. This showed that an acidified extraction process used at the first stage of the purification procedure is able to remove the toxic alkaloids from the crude drug. The LC-MS/MS analyses showed that the main components of JG preparations were fatty acids and apigenin glycosides; it seemed that the fatty acids can be used for enhancing the dissolution of the polar glycosides. T-test calculation using Profile Analysis software showed that the acidified crude drugs, extract, granules of JG, and gendarussa capsules showed very similar LC-MS/MS profiles, which means that the biochemical components of JG are relatively stable during processing. Due to the lack of quality markers for these JG preparations, the application of metabolite profiling is recommended as the QC tool for commercial production by the pharmaceutical industry.
KW - Alkaloids
KW - Apigenin glycosides
KW - Extract of gendarussa
KW - Gendarussa capsules
KW - Justicia gendarussa
KW - LC MS/MS profiling
UR - http://www.scopus.com/inward/record.url?scp=85067398154&partnerID=8YFLogxK
U2 - 10.1177/1934578X19851406
DO - 10.1177/1934578X19851406
M3 - Article
AN - SCOPUS:85067398154
SN - 1934-578X
VL - 14
JO - Natural Product Communications
JF - Natural Product Communications
IS - 6
ER -