Phytochemical Analysis and Molecular Identification of Green Macroalgae Caulerpa spp. from Bali, Indonesia

I. Gede Putu Wirawan, Ni Kadek Emi Sintha Dewi, Maria Malida Vernandes Sasadara, I. Gde Nengah Adhilaksman Sunyamurthi, I. Made Jawi, I. Nyoman Wijaya, Ida Ayu Putri Darmawati, I. Ketut Suada, Anak Agung Keswari Krisnandika

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


The studies of the Bulung Boni and Bulung Anggur (Caulerpa spp.) species and secondary metabolites are still very limited. Proper identification will support various aspects, such as cultivation, utilization, and economic interests. Moreover, understanding the secondary metabolites will assist in developing algae-based products. This study aimed to identify these indigenous Caulerpa algae and analyze their bioactive components. The tufA sequence was employed as a molecular marker in DNA barcoding, and its bioactive components were identified using the GC-MS method. The phylogenetic tree was generated in MEGA 11 using the maximum likelihood method, and the robustness of the tree was evaluated using bootstrapping with 1000 replicates. This study revealed that Bulung Boni is strongly connected to Caulerpa cylindracea. However, Bulung Anggur shows no close relationship to other Caulerpa species. GC-MS analysis of ethanolic extracts of Bulung Boni and Bulung Anggur showed the presence of 11 and 13 compounds, respectively. The majority of the compounds found in these algae have been shown to possess biological properties, such as antioxidant, antibacterial, anticancer, anti-inflammation, and antidiabetic. Further study is necessary to compare the data obtained using different molecular markers in DNA barcoding, and to elucidate other undisclosed compounds in these Caulerpa algae.

Original languageEnglish
Article number4879
Issue number15
Publication statusPublished - Aug 2022
Externally publishedYes


  • DNA barcoding
  • GC-MS
  • molecular identification
  • molecular marker
  • phylogeny
  • phytochemical
  • tufA


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