TY - JOUR
T1 - Phytochemical analysis and antibacterial activity of purple leaf extract [graptophyllum pictum (l.) griff] against streptococcus mutans
AU - Juniarti, Devi Eka
AU - Kusumaningsih, Tuti
AU - Juliastuti, Wisnu Setyari
AU - Soetojo, Adioro
AU - Wungsu, Nabilla Dara
N1 - Funding Information:
This paper was funded by the University of Airlangga.
Publisher Copyright:
© 2021 University of the Philippines Manila. All rights reserved.
PY - 2021/11
Y1 - 2021/11
N2 - Background. Streptococcus mutans is the leading cause of dental caries. One of many medicinal plants, purple leaf [Graptophyllum pictum (L.) Griff], which contains flavonoids, alkaloids, tannins, steroids, and saponins, is a potential antibacterial agent. Objective. This study aimed to determine the antibacterial activity of purple leaf extract (Graptophyllum pictum L. Griff) against Streptococcus mutans. Methods. Streptococcus mutans were suspended in several Graptophyllum pictum (L.) Griff extract concentrations in a BHIB medium using the dilution method so that the concentration of 100%, 50%, 25%, 12.5%, 6.25%, 3.12%, 1.56%, 0.78% were obtained. Each tube was incubated for 24 hours, then subcultured in a Tryptone Yeast Extract Cystine medium in a petri dish using a spreader. Each petri dish was set for 24 hours; the growth of the colony, using CFU/mL unit, was manually calculated. The samples were then subjected to microbiological analysis. The Tukey's Honest Significant Difference test was performed to determine if the relationship between the sets of data in the treatment group is statistically significant (p<0.05). Results. Purple leaf extract contains bioactive compounds such as flavonoid, alkaloid, tannin, triterpenoid/ steroid, and saponin. The Minimum Inhibitory Concentration (MIC) of Graptophyllum pictum (L.) Griff against Streptococcus mutans was in concentration 3.125%, and the Minimum Bactericidal Concentration (MBC) was in concentration 6.25%. Conclusion. Purple Leaf Extract [Graptophyllum pictum (L.) Griff] has antibacterial activity against Streptococcus mutans.
AB - Background. Streptococcus mutans is the leading cause of dental caries. One of many medicinal plants, purple leaf [Graptophyllum pictum (L.) Griff], which contains flavonoids, alkaloids, tannins, steroids, and saponins, is a potential antibacterial agent. Objective. This study aimed to determine the antibacterial activity of purple leaf extract (Graptophyllum pictum L. Griff) against Streptococcus mutans. Methods. Streptococcus mutans were suspended in several Graptophyllum pictum (L.) Griff extract concentrations in a BHIB medium using the dilution method so that the concentration of 100%, 50%, 25%, 12.5%, 6.25%, 3.12%, 1.56%, 0.78% were obtained. Each tube was incubated for 24 hours, then subcultured in a Tryptone Yeast Extract Cystine medium in a petri dish using a spreader. Each petri dish was set for 24 hours; the growth of the colony, using CFU/mL unit, was manually calculated. The samples were then subjected to microbiological analysis. The Tukey's Honest Significant Difference test was performed to determine if the relationship between the sets of data in the treatment group is statistically significant (p<0.05). Results. Purple leaf extract contains bioactive compounds such as flavonoid, alkaloid, tannin, triterpenoid/ steroid, and saponin. The Minimum Inhibitory Concentration (MIC) of Graptophyllum pictum (L.) Griff against Streptococcus mutans was in concentration 3.125%, and the Minimum Bactericidal Concentration (MBC) was in concentration 6.25%. Conclusion. Purple Leaf Extract [Graptophyllum pictum (L.) Griff] has antibacterial activity against Streptococcus mutans.
KW - Antibacterial
KW - Medicine
KW - Phytochemical
KW - Streptococcus mutans
UR - http://www.scopus.com/inward/record.url?scp=85120944979&partnerID=8YFLogxK
U2 - 10.47895/AMP.V55I8.2125
DO - 10.47895/AMP.V55I8.2125
M3 - Article
AN - SCOPUS:85120944979
SN - 0001-6071
VL - 55
SP - 802
EP - 806
JO - Acta Medica Philippina
JF - Acta Medica Philippina
IS - 8
ER -