TY - JOUR
T1 - Physiological study of the use of bull seminal plasma in skim milk diluent to improve quality of frozen ram semen
AU - Susilowati, Suherni
AU - Wurlina,
AU - Dewa Putu Anom Adnyana, I.
AU - Mustofa, Imam
AU - Hariadi, Mas’Ud
N1 - Publisher Copyright:
© 2019 Susilowati et al.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Ram semen freezing process still generates poor outcome due to phospholipase. Thus, an alternative to substitute ram seminal plasma is recently developed using bull seminal plasma. The aim of this study was to determine the benefits of bull seminal plasma added by ram spermatozoa diluent in the freezing process. This study used control treatment (P0); (P1) was (diluent + ram semen) + bull seminal plasma (1: 1); (P2) was (diluent + ram semen) + bull seminal plasma (1:2). The result showed that P1 generated the highest viability, motility, plasma membrane intact, and the lowest DNA fragmentation, compared to second treatment and control. Statistical analysis of viability, motility, plasma membrane intact and DNA fragmentation were significantly different (p<0.05) among treatments. It can be concluded that additional of seminal plasma of bull can maintain motility, viability, plasma membrane intact and decrease the of DNA fragmentation in ram spermatozoa after-thawing.
AB - Ram semen freezing process still generates poor outcome due to phospholipase. Thus, an alternative to substitute ram seminal plasma is recently developed using bull seminal plasma. The aim of this study was to determine the benefits of bull seminal plasma added by ram spermatozoa diluent in the freezing process. This study used control treatment (P0); (P1) was (diluent + ram semen) + bull seminal plasma (1: 1); (P2) was (diluent + ram semen) + bull seminal plasma (1:2). The result showed that P1 generated the highest viability, motility, plasma membrane intact, and the lowest DNA fragmentation, compared to second treatment and control. Statistical analysis of viability, motility, plasma membrane intact and DNA fragmentation were significantly different (p<0.05) among treatments. It can be concluded that additional of seminal plasma of bull can maintain motility, viability, plasma membrane intact and decrease the of DNA fragmentation in ram spermatozoa after-thawing.
KW - After-thawing
KW - Bull seminal plasma
KW - DNA fragmentation of spermatozoa
KW - Freezing process
KW - Ram semen
UR - http://www.scopus.com/inward/record.url?scp=85069842312&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85069842312
SN - 1307-9867
VL - 13
SP - 193
EP - 197
JO - EurAsian Journal of BioSciences
JF - EurAsian Journal of BioSciences
IS - 1
ER -