PHYLOGENETIC ANALYSIS AND MUTATION OF SARS-COV-2 IN BATS IN KARST MALANG CITY, INDONESIA

Soedjajadi Keman, Moch Irfan Hadi, Dedy Suprayogi, Yudied Agung Mirasa

Research output: Contribution to journalArticlepeer-review

Abstract

Introduction: A group of people in China were hospitalized with an initial diagnosis of pneumonia of unknown cause. The patients were linked to a wholesale wet seafood and animal market in Wuhan, Hubei Province, China. The disease has spread to other provinces in China, Thailand, Japan, and South Korea in less than a month. SARS-CoV-2 was found to originate from bats. Therefore, this research aims to analyze SARS-CoV-2 mutation in bats in Malang Karst, Indonesia. Methods: Other bat body parts used as research samples include the brain, liver, kidneys, intestines, pancreas, fetus, blood, lungs, and ectoparasites. The samples were taken separately and placed in a container containing 10% PBF. For further analysis, we used RNA Extraction, Real-Time PCR, Sequencing, and CoV Gisaid mutation analysis software to analyze the sequencing data. Then, EMBL software will be used to analyze the phylogenetically. Results and Discussion: There was 1 sample that showed a positive result for Covid-19, namely the intestine of the Cynoptera brachyotis species. There were differences between SARS-CoV-2 in bats in Malang Karst in Indonesia compared to SARS-CoV from 2000 to 2019. The spike protein's receptor binding domain (RBD) is the most variable part of the coronavirus genome. Conclusion: From the research results, one positive sample was obtained using Real-Time PCR, and based on mutation analysis, mutations were found in SARS-CoV-2 against the SARS-CoV virus from 2000-2019. Further research is needed, especially regarding SARS-CoV-2 as a vaccine.

Original languageEnglish
Pages (from-to)173-180
Number of pages8
JournalJurnal Kesehatan Lingkungan
Volume16
Issue number2
DOIs
Publication statusPublished - 29 Apr 2024

Keywords

  • Bats
  • Mutation
  • Phylogenetic analysis
  • SARS-CoV-2

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