Photodynamic potential of blue diode laser inactivation with chlorophyll photosensitisers in Pseudomonas aeruginosa and Staphylococcus aureus bacteria

M. Aulia Iwinda, A. Suryani Dyah, Samian Samian

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Photodynamic Inactivation (PDI) research has been conducted as a method of photodynamic therapy that combines blue diode laser light (405 nm) with Alfalfa chlorophyll photosensitiser so that it can produce reactive oxygen species that cause biological damage to the target. This study aims to determine the potential of blue diode lasers and the addition of 20% chlorophyll photosensitiser to reduce gram-positive Staphylococcus aureus and gram-negative Pseudomonas aeruginosa which can cause skin infections. The method used is a blue diode laser irradiation of bacteria by the addition of chlorophyll and Total Plate Count (TPC) to determine the decrease in bacterial viability in units of CFU / ml. Test results show that photodynamic inactivation with a blue diode laser and chlorophyll can reduce bacterial viability. Irradiation for 180 seconds at a dose of 1.59 J / cm2 gives the most bacterial death results. The percentage of bacterial death of Staphylococcus aureus was (35.44 ± 1.67)% without photosensitiser, and with photosensitiser the percentage of bacterial deaths increased to (53.59 ± 1.36)%. While the percentage of Pseudomonas aeruginosa bacterial deaths was (20.02 ± 0.76)% without photosensitisers, and with additional photosensitisers it increased to (44.24 ± 1.50)%.

Original languageEnglish
Title of host publicationFourth International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2020
EditorsAgus Muhammad Hatta, Aulia Nasution, Ruri Agung Wahyuono
PublisherSPIE
ISBN (Electronic)9781510644120
DOIs
Publication statusPublished - 2021
Event4th International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2020 - Virtual, Online, Indonesia
Duration: 1 Dec 20202 Dec 2020

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume11789
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

Conference4th International Seminar on Photonics, Optics, and Its Applications, ISPhOA 2020
Country/TerritoryIndonesia
CityVirtual, Online
Period1/12/202/12/20

Keywords

  • Laser Diode
  • Photoinactivation
  • Photosensitizer
  • Pseudomonas aeruginosa
  • Staphylococcus aureus

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