Photodynamic Inactivation of Streptococcus mutan Bacteri with Photosensitizer Moringa oleifera Activated by Light Emitting Diode (LED)

A. I. Mardianto, E. M. Setiawatie, W. P. Lestari, A. Rasheed, S. D. Astuti

Research output: Contribution to journalConference articlepeer-review

10 Citations (Scopus)

Abstract

A Photodynamic Inactivation (PDI) research has been done as a method of photodynamic in a medical treatment that combine light with photosensitizer. The light energy absorption by the photosensitizer molecules will produce reactive oxygen species that cause biological damage. Photoinactivation has done by combining blue LED light and exogenous porphyrin photosensitizer Moringa oleifera leaf extract to a major cause of dental caries disease, Streptococcus mutan. The goal of this research is to analize a maximum potential of LED exposure angle to inactivates a Streptococcus mutan bacteria added by 20% concentration of Moringa oleifera leaf extract photosensitizer. The wavelength of blue LED light used is 450.00 ± 0.21 nm in 100% Pulse Width Modulation (PWM). Total Plate Count (TPC) is used as a method to determine the loss of bacteria viability in unit CFU/ml. The stastical test result show an angle that potentially inactivate the bacteria by the exposure of two groups treatment with six variation of angle and an exposure time is 90° angle which has different meaning with another variation (p < a = 0.05), that means the best angle to use is perpendicular which has a percentage result of absorbent media for 180 second duration is (30,301 ± 4,231)% of bacterial death without photosensitizer and increased to (46,742 ± 1,667)% bacterial death with a photosensitizer.

Original languageEnglish
Article number012061
JournalJournal of Physics: Conference Series
Volume1505
Issue number1
DOIs
Publication statusPublished - 15 Jun 2020
Event3rd Annual Scientific Meeting on Medical Physics and Biophysics, PIT-FMB in conjunction with the 17th South-East Asia Congress of Medical Physics, SEACOMP 2019 - Bali, Indonesia
Duration: 8 Aug 201910 Aug 2019

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