TY - GEN
T1 - Photodynamic inactivation effect of laser diode on Staphylococcus aureus bacteria with exogenous photosensitizer nano doxycycline 0.1%
AU - Astuti, Suryani Dyah
AU - Risma, Dian Ayu
AU - Zaidan, Andi Hamim
AU - Puspita, Putri Suryaning
N1 - Publisher Copyright:
© 2020 Author(s).
PY - 2020/12/9
Y1 - 2020/12/9
N2 - Infection is the entry of bacteria or pathogenic microorganisms into the body that can cause pain. For example, Staphylococcus aureus infection is caused by direct contamination of wounds, either post-surgical or infection after trauma. This study aims to determine the effectiveness of nano doxycycline as an exogenous photosensitizer agent in antimicrobial photodynamic inactivation (PDI) by using laser diode activator (403±0.02) nm against Staphylococcus aureus bacteria. The study was divided into three groups which were T0 as the untreated control group, T1 as treatment group with nano doxycyclin but without laser exposure, T2 as Treatment group with nano doxycyclin and laser treatments at various exposure times (30, 60, 90, 120, 150) seconds. This study used the Total Plate Count (TPC) method to find out the percentage decrease in the number of colonies. The results of statistical analysis showed that there were significant differences in the number of bacterial colonies due to the addition of nano doxycycline 0.1% and laser diode exposure (403.00±0.02) nm at (150.00±0.01) second with a energy density of (21.83±0.02) J/cm2. The results showed the addition of nano doxycycline was effective to reduce the number of bacterial colonies with a percentage of deaths of (85.34±1.20)%. So, antimicrobial photodynamic inactivation using laser diode with nano doxycyclin is effective to reduce the number of S. aureus bacteria.
AB - Infection is the entry of bacteria or pathogenic microorganisms into the body that can cause pain. For example, Staphylococcus aureus infection is caused by direct contamination of wounds, either post-surgical or infection after trauma. This study aims to determine the effectiveness of nano doxycycline as an exogenous photosensitizer agent in antimicrobial photodynamic inactivation (PDI) by using laser diode activator (403±0.02) nm against Staphylococcus aureus bacteria. The study was divided into three groups which were T0 as the untreated control group, T1 as treatment group with nano doxycyclin but without laser exposure, T2 as Treatment group with nano doxycyclin and laser treatments at various exposure times (30, 60, 90, 120, 150) seconds. This study used the Total Plate Count (TPC) method to find out the percentage decrease in the number of colonies. The results of statistical analysis showed that there were significant differences in the number of bacterial colonies due to the addition of nano doxycycline 0.1% and laser diode exposure (403.00±0.02) nm at (150.00±0.01) second with a energy density of (21.83±0.02) J/cm2. The results showed the addition of nano doxycycline was effective to reduce the number of bacterial colonies with a percentage of deaths of (85.34±1.20)%. So, antimicrobial photodynamic inactivation using laser diode with nano doxycyclin is effective to reduce the number of S. aureus bacteria.
UR - http://www.scopus.com/inward/record.url?scp=85097977502&partnerID=8YFLogxK
U2 - 10.1063/5.0034129
DO - 10.1063/5.0034129
M3 - Conference contribution
AN - SCOPUS:85097977502
T3 - AIP Conference Proceedings
BT - 2nd International Conference on Physical Instrumentation and Advanced Materials 2019
A2 - Trilaksana, Herri
A2 - Harun, Sulaiman Wadi
A2 - Shearer, Cameron
A2 - Yasin, Moh
PB - American Institute of Physics Inc.
T2 - 2nd International Conference on Physical Instrumentation and Advanced Materials, ICPIAM 2019
Y2 - 22 October 2019
ER -