Pengaruh Konsentrasi Gliserol dalam Pengencer Tris Terhadap Kualitas Spermatozoa Kambing Sapera Before Freezing

This study aimed to determine the optimal glycerol concentration in the tris dilution to maintain the spermatozoa quality of Sapera goats. This study used 1.5 year old male Sapera goats. The glycerol concentration used 5%, 6% and 7%. Semen was collected twice a week using an artificial vagina. The results showed the average percentage of motility and viability of spermatozoa in diluents with glycerol 6% was 62.33% and 75.83%, respectively; there were significant differences (p<0.05) compared to diluents with glycerol 5% was 57.67% and 68.33%, respectively; and 7% was 59% and 70.33%, respectively. Average abnormalities in diluents with glycerol 5%, 6% and 7% were 8%, 7.5% and 7.67%, respectively; there were no significant differences (p>0.05). It can be concluded that the addition of 6% glycerol in Tris diluents succeeded in protecting spermatozoa from cold stress during the glycerolization process, so as to maintain the motility, viability and abnormalities of spermatozoa.