Peneguhan Diagnosis Penyakit Inclusion Bodies Hepatitis pada Ayam Broiler Menggunakan Metode Polymerase Chain Reaction

Translated title of the contribution: Diagnosis Confirmation of Inclusion Bodies Hepatitis on Broiler Chicken using Polymerase Chain Reaction Method

Jola Rahmahani, Maha Kirana, Fedik Abdul Rantam, Suwarno

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

This study aimed to diagnose cases of Inclusion Bodies Hepatitis (IBH) in broiler chickens. A total of 6 chickens were collected from farms in Banten and Jember. Samples were taken from the liver which underwent changes such as brittle, yellowish in color and enlarged. The samples were ground and extracted, then the Polymerase Chain Reaction (PCR) test was performed using Primers Hexon A (5'-CAARTTCAGRCAGACGGT-3') and Hexon B (5'-TAGTGATGMCGSGACATCAT-3') with 897 bp length. The results showed that the broiler chicken case sample from Banten was positively 100% infected by the IBH Virus.

Translated title of the contributionDiagnosis Confirmation of Inclusion Bodies Hepatitis on Broiler Chicken using Polymerase Chain Reaction Method
Original languageIndonesian
Pages (from-to)145-148
Number of pages4
JournalJurnal Medik Veteriner
Volume5
Issue number2
DOIs
Publication statusPublished - 1 Oct 2022

Keywords

  • Inclusion Bodies Hepatitis
  • Polymerase Chain Reaction
  • broiler

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