TY - JOUR
T1 - p16INK4A Expression in Condyloma Acuminata Lesions Associated with High-Risk Human Papillomavirus Infection
AU - Mastutik, Gondo
AU - Rahniayu, Alphania
AU - Arista, Afria
AU - Murtiastutik, Dwi
AU - Kurniasari, Nila
AU - Setyaningrum, Trisniartami
AU - Rahaju, Anny Setijo
AU - Sulistyani, Erna
N1 - Funding Information:
We are thankful of Government of the Republic of Indonesia, the Ministry of Research, Technology and Higher Education, and the Faculty of Medicine, Airlangga University for funding in this research with the university’s operational funding for the 2017 fiscal year.
Funding Information:
We are thankful of Government of the Republic of Indonesia, the Ministry of Research, Technology and Higher Education, and the Faculty of Medicine, Airlangga University for funding in this research with the university?s operational funding for the 2017 fiscal year. Funding statement This study was supported by the Faculty of Medicine, Airlangga University, and the university?s operational funding for the 2017 fiscal year. This manuscript have not been presented at a scientific event or submitted to any other journals. Ethical statement Ethical study was approved by the Medical Ethic Research at Dr. Soetomo General Academic Hospital Surabaya, Indonesia (ethical code:382/Panke. KKE/V/2016). Statement conflict of interest The authors declare no conflict of interest
Funding Information:
This study was supported by the Faculty of Medicine, Airlangga University, and the university’s operational
Publisher Copyright:
© 2021. All Rights Reserved.
PY - 2021
Y1 - 2021
N2 - Objective: The objective of this study was to discover the possible correlation between p16INK4A expression and the LR/HR-HPV infection in condyloma acuminate (CA) lesions. Materials and Method: This cross-sectional study was conducted during January-December 2017 on 33 CA patients. The expression of p16INK4A was detected by immunohistochemistry (IHC) staining. The positive interpretation was carried out by scoring which score 0 was negative, score 1 was sporadic, score 2 was focal, and score 3 was diffuses. The HPV genotypes were identified by reverse line blot, and 40 genotypes of HPV detected, including HR-HPV (HPVs 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 67, 68a, 68b, 69, 73, and 82) and LR-HPV (HPVs 6, 11, 40, 42, 43, 44, 54, 55, 61, 62, 64, 70, 71, 72, 81, 83, 84, 87, 89, and 90). Results: The expression of p16INK4A was significantly correlated with HR-HPV infection. Patients infected with HR-HPV had 0.644 times higher possibility to express p16INK4A gene compared to those infected with LR-HPV. LR-HPV genotypes detected in CA patients were HPVs 6, 11, 42, 61, 54, 81, 87, 89, and 90 and HR-HPV genotypes were HPVs 18, 26, 45, 51, 52, 67, 68B, 69, and 82. LR-HPV was found in 19/33 of patients and HR-HPV was in 14/33 of patients. The expression of p16INK4A in CA lesions was diffuse in15.2% of patients, was focal in 24.2% of patients, was sporadic in 39.4% of patients were, and was negative in 21.2% of patients. In LR-HPV group, there was no diffuse expression, focal expression was observed in 15.8%, sporadic in 47.4%, and negative in 36.8%, while in HR-HPV group, p16INK4A expression was detected in all lesions, in a way that its expression was diffuse in 35.7%, focal in 35.7%, and sporadic in 28.6%. Conclusion: IHC is a routine method in histopathological diagnosis, therefore the detection of p16INK4A expression by IHC can be used as a biomarker for HR-HPV infection diagnosis.
AB - Objective: The objective of this study was to discover the possible correlation between p16INK4A expression and the LR/HR-HPV infection in condyloma acuminate (CA) lesions. Materials and Method: This cross-sectional study was conducted during January-December 2017 on 33 CA patients. The expression of p16INK4A was detected by immunohistochemistry (IHC) staining. The positive interpretation was carried out by scoring which score 0 was negative, score 1 was sporadic, score 2 was focal, and score 3 was diffuses. The HPV genotypes were identified by reverse line blot, and 40 genotypes of HPV detected, including HR-HPV (HPVs 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 67, 68a, 68b, 69, 73, and 82) and LR-HPV (HPVs 6, 11, 40, 42, 43, 44, 54, 55, 61, 62, 64, 70, 71, 72, 81, 83, 84, 87, 89, and 90). Results: The expression of p16INK4A was significantly correlated with HR-HPV infection. Patients infected with HR-HPV had 0.644 times higher possibility to express p16INK4A gene compared to those infected with LR-HPV. LR-HPV genotypes detected in CA patients were HPVs 6, 11, 42, 61, 54, 81, 87, 89, and 90 and HR-HPV genotypes were HPVs 18, 26, 45, 51, 52, 67, 68B, 69, and 82. LR-HPV was found in 19/33 of patients and HR-HPV was in 14/33 of patients. The expression of p16INK4A in CA lesions was diffuse in15.2% of patients, was focal in 24.2% of patients, was sporadic in 39.4% of patients were, and was negative in 21.2% of patients. In LR-HPV group, there was no diffuse expression, focal expression was observed in 15.8%, sporadic in 47.4%, and negative in 36.8%, while in HR-HPV group, p16INK4A expression was detected in all lesions, in a way that its expression was diffuse in 35.7%, focal in 35.7%, and sporadic in 28.6%. Conclusion: IHC is a routine method in histopathological diagnosis, therefore the detection of p16INK4A expression by IHC can be used as a biomarker for HR-HPV infection diagnosis.
KW - p16- high-risk HPV- low-risk HPV- condyloma- sexually transmitted diseases
UR - http://www.scopus.com/inward/record.url?scp=85118766585&partnerID=8YFLogxK
U2 - 10.31557/APJCP.2021.22.10.3219
DO - 10.31557/APJCP.2021.22.10.3219
M3 - Article
AN - SCOPUS:85118766585
SN - 1513-7368
VL - 22
SP - 3219
EP - 3225
JO - Asian Pacific Journal of Cancer Prevention
JF - Asian Pacific Journal of Cancer Prevention
IS - 10
ER -
