TY - GEN
T1 - Oxidative stress responses in gills of tilapia (Oreochromis niloticus) at different salinities
AU - Handayani, Kiki Syaputri
AU - Novianty, Zahra
AU - Saputri, Miftahul Rohmah
AU - Irawan, Bambang
AU - Soegianto, Agoes
N1 - Publisher Copyright:
© 2017 Author(s).
PY - 2017/8/4
Y1 - 2017/8/4
N2 - The objective of present study is to evaluate the impact of different salinities on the levels of CAT, GSH and MDA of the gills of Nile tilapia (Oreochromis niloticus). Nile tilapia was treated by exposure to salinities concentration 0 ‰, 5 ‰ and 10 ‰. Research models were weakened and sacrificed, then took the left and right sides of the gills. The result of gills homogenity was centrifuged for supernatan, then supernatan was proceed with testing levels of CAT, GSH and MDA by ELISA assay methods. The levels of CAT in gills were significantly higher at 10 ‰ than at 5 ‰ and 0 ‰. The levels of GSH in gills were significantly higher at 0 ‰ than 5 ‰. The levels of GSH in gills at 5 ‰ and 10 ‰ salinities were not significantly different. The levels of MDA in gills at salinity 10 ‰ and 5 ‰ were higher than in control gills at 0 ‰ salinities. This occurs because the salinity of 10 ‰ salinity was optimal for live of fish tilapia. In conclusion, salinity impact the increasing of CAT, GSH, and MDA levels in gills of Nile tilapia.
AB - The objective of present study is to evaluate the impact of different salinities on the levels of CAT, GSH and MDA of the gills of Nile tilapia (Oreochromis niloticus). Nile tilapia was treated by exposure to salinities concentration 0 ‰, 5 ‰ and 10 ‰. Research models were weakened and sacrificed, then took the left and right sides of the gills. The result of gills homogenity was centrifuged for supernatan, then supernatan was proceed with testing levels of CAT, GSH and MDA by ELISA assay methods. The levels of CAT in gills were significantly higher at 10 ‰ than at 5 ‰ and 0 ‰. The levels of GSH in gills were significantly higher at 0 ‰ than 5 ‰. The levels of GSH in gills at 5 ‰ and 10 ‰ salinities were not significantly different. The levels of MDA in gills at salinity 10 ‰ and 5 ‰ were higher than in control gills at 0 ‰ salinities. This occurs because the salinity of 10 ‰ salinity was optimal for live of fish tilapia. In conclusion, salinity impact the increasing of CAT, GSH, and MDA levels in gills of Nile tilapia.
UR - http://www.scopus.com/inward/record.url?scp=85028320129&partnerID=8YFLogxK
U2 - 10.1063/1.4995198
DO - 10.1063/1.4995198
M3 - Conference contribution
AN - SCOPUS:85028320129
T3 - AIP Conference Proceedings
BT - 4th International Conference on Research, Implementation, and Education of Mathematics and Science, 4TH ICRIEMS
A2 - Suyanto, Slamet
A2 - Kusumawardani, Cahyorini
A2 - Wilujeng, Insih
A2 - Abadi, Agus Maman
PB - American Institute of Physics Inc.
T2 - 4th International Conference on Research, Implementation, and Education of Mathematics and Sciences: Research and Education for Developing Scientific Attitude in Sciences and Mathematics, ICRIEMS 2017
Y2 - 15 May 2017 through 16 May 2017
ER -