Osteogenesis at tension site by Stichopus hermanii application as relapse orthodontic prevention

Objective: The aim of this study is to investigate osteogenesis at tension site by Stichopus hermanii application as relapse orthodontic prevention. Materials and Methods: The experiment was held by Post Test Only Group design. Thirty two male Cavia Cobaya were divided into four groups. K(-) group as negative control group (without treatment), K(+) group as positive control group which were applied with relaps orthodontic forces, and the other groups P1, P2, were applied with relaps orthodontic forces and Stichopus hermanii 2,5% and 3%. After treatment the cavia cobaya were sacrificed. Collagen type1 and BMP-2 expression at tension site as osteogenesis marker were examined with immunohistochemistry. Results: This study showed collagen type 1 and BMP-2 expression especially in P2 increased to show osteogenesis occured compare with K(-), K(+), and P1. Conclusion: Osteogenesis occurred at tension site by Stichopus hermanii as relapse orthodontic prevention. 3% Stichopus hermanii is the effective dose for osteogenesis.