TY - JOUR
T1 - Optimization of ultrasound-assisted extraction and the antioxidant activities of Sidaguri (Sida rhombifolia)
AU - Ikhtiarini, Asefin Nurul
AU - Setyaningsih, Widiastuti
AU - Rafi, Mohamad
AU - Aminah, Nanik Siti
AU - Insanu, Muhamad
AU - Imawati, Irnawati
AU - Rohman, Abdul
N1 - Funding Information:
This study was supported by the Program of Riset Kolaborasi Indonesia (RKI), Directorate of Research, Universitas Gadjah Mada, Indonesia, 2021.
Publisher Copyright:
© 2021 Asefin Nurul Ikhtiarini et al. All Rights Reserved.
PY - 2021/8
Y1 - 2021/8
N2 - Sidaguri (Sida rhombifolia) is widely distributed in tropical and subtropical regions and has been reported to possess many bioactive compounds that are beneficial for human health, including polyphenolics having antioxidant activities. The objective of this study was (1) to optimize ultrasound-assisted extraction (UAE) of Sidaguri and (2) to evaluate antioxidant activities of extracts resulting from the optimized condition using radical scavenging assay of 2,2‘-diphenil-1-picrylhydrazyl (DPPH) and 2,2‘-azinobis-3-ethylbenzothiazolme-6-sulfomc acid. The optimum UAE conditions obtained were solvent to solid ratio of 26:1, temperature of extraction of 45.45°C, methanol concentration of 42%, power of sonication of 86%, and time of extraction of 5 minutes. Sidaguri obtained from locations of Kretek and Ngemplak had the highest radical scavenging activity accounting for 83.69% ± 0.30% and 82.95% ± 0.49%, respectively.
AB - Sidaguri (Sida rhombifolia) is widely distributed in tropical and subtropical regions and has been reported to possess many bioactive compounds that are beneficial for human health, including polyphenolics having antioxidant activities. The objective of this study was (1) to optimize ultrasound-assisted extraction (UAE) of Sidaguri and (2) to evaluate antioxidant activities of extracts resulting from the optimized condition using radical scavenging assay of 2,2‘-diphenil-1-picrylhydrazyl (DPPH) and 2,2‘-azinobis-3-ethylbenzothiazolme-6-sulfomc acid. The optimum UAE conditions obtained were solvent to solid ratio of 26:1, temperature of extraction of 45.45°C, methanol concentration of 42%, power of sonication of 86%, and time of extraction of 5 minutes. Sidaguri obtained from locations of Kretek and Ngemplak had the highest radical scavenging activity accounting for 83.69% ± 0.30% and 82.95% ± 0.49%, respectively.
KW - antioxidant activities
KW - Box-Behnken design
KW - Sidaguri
KW - ultrasound-assisted extraction
UR - http://www.scopus.com/inward/record.url?scp=85112809721&partnerID=8YFLogxK
U2 - 10.7324/JAPS.2021.110810
DO - 10.7324/JAPS.2021.110810
M3 - Article
AN - SCOPUS:85112809721
SN - 2231-3354
VL - 11
SP - 70
EP - 76
JO - Journal of Applied Pharmaceutical Science
JF - Journal of Applied Pharmaceutical Science
IS - 8
ER -
