TY - JOUR
T1 - Optimization of ionic liquid-microwave assisted extraction method of Mahkota Dewa (Phaleria macrocarpa (Scheff.) Boerl.) fruit pulp
AU - Handayani, Rosita
AU - Purnamasari, Wulan
AU - Mun'im, Abdul
N1 - Publisher Copyright:
© 2021 Rosita Handayani et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
PY - 2021/2
Y1 - 2021/2
N2 - Mahkota dewa fruit pulp (Phaleria macrocarpa, PMp) is often used as a medicinal raw material in Indonesia. The texture of the P. macrocarpa fruit is hard due to high lignin and cellulose contents and it often inhibits an extraction process of secondary metabolism. This study is conducted to get optimum extraction condition by using the ionic liquid-microwave assisted extraction (IL-MAE) method, assessing the effect of pretreatment with urea and the antioxidant activity of PMp. Solvent [BMIM]BF4 has been chosen for extraction by varying three parameters: solvent concentration, extraction time, and solvent–sample ratio. The optimum condition was determined based on total phenolic content (TPC; milligram equivalent to quercetin as reference), antioxidant activity assay has been carried out using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method, and selectivity to extract polar compound was carried out using High-Performance Liquid Chromatography (HPLC) method. The optimum extraction condition has been achieved at run 11 with the condition: 10% microwave power, using [BMIM]BF4 (2.5 M) as a solvent, using 0.01 M KH2PO4 as a salt solution, 2.5 minutes extraction time, solvent: sample ratio (17.5: 1), resulting TPC value of 191.72 ± 1.27 mgGAE/g powder, and DPPH radical-scavenging activity of 12.1 ± 0.003%. The IL-MAE method can extract the marker of phenolic compounds (quercetin, mahkoside, benzophenone, and mangiferin) and showed selectivity in extracting polar compound than conventional methods. Pretreatment using 8% urea for 12 hours produced no significant difference in TPC values from the IL-MAE method at optimum conditions. The IL-MAE method gives the highest TPC and superior antioxidant activity in PMp extraction.
AB - Mahkota dewa fruit pulp (Phaleria macrocarpa, PMp) is often used as a medicinal raw material in Indonesia. The texture of the P. macrocarpa fruit is hard due to high lignin and cellulose contents and it often inhibits an extraction process of secondary metabolism. This study is conducted to get optimum extraction condition by using the ionic liquid-microwave assisted extraction (IL-MAE) method, assessing the effect of pretreatment with urea and the antioxidant activity of PMp. Solvent [BMIM]BF4 has been chosen for extraction by varying three parameters: solvent concentration, extraction time, and solvent–sample ratio. The optimum condition was determined based on total phenolic content (TPC; milligram equivalent to quercetin as reference), antioxidant activity assay has been carried out using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method, and selectivity to extract polar compound was carried out using High-Performance Liquid Chromatography (HPLC) method. The optimum extraction condition has been achieved at run 11 with the condition: 10% microwave power, using [BMIM]BF4 (2.5 M) as a solvent, using 0.01 M KH2PO4 as a salt solution, 2.5 minutes extraction time, solvent: sample ratio (17.5: 1), resulting TPC value of 191.72 ± 1.27 mgGAE/g powder, and DPPH radical-scavenging activity of 12.1 ± 0.003%. The IL-MAE method can extract the marker of phenolic compounds (quercetin, mahkoside, benzophenone, and mangiferin) and showed selectivity in extracting polar compound than conventional methods. Pretreatment using 8% urea for 12 hours produced no significant difference in TPC values from the IL-MAE method at optimum conditions. The IL-MAE method gives the highest TPC and superior antioxidant activity in PMp extraction.
KW - Antioxidant
KW - BF4
KW - IL-MAE
KW - Mahkota dewa
KW - Phaleria macrocarpa
KW - ionic liquid
KW - microwave
UR - http://www.scopus.com/inward/record.url?scp=85101560744&partnerID=8YFLogxK
U2 - 10.7324/JAPS.2021.110208
DO - 10.7324/JAPS.2021.110208
M3 - Article
AN - SCOPUS:85101560744
SN - 2231-3354
VL - 11
SP - 59
EP - 65
JO - Journal of Applied Pharmaceutical Science
JF - Journal of Applied Pharmaceutical Science
IS - 2
ER -